植物遗传资源学报2026，Vol.27Issue(4)：789-802,14.DOI:10.13430/j.cnki.jpgr.20251111001
基于水培技术的甘薯种质弱苗复壮方法研究
Research on Hydroponic Rejuvenation Technology for Weak Seedlings of Sweet Potato Germplasm Resources
摘要
Abstract
The secure preservation of germplasm resources serves as a critical foundation for crop breeding,and developing efficient conservation methods for weak-growth accessions is particularly crucial.This study utilized 135 accessions of weak sweet potato seedlings as experimental materials.These materials were hydroponically cultured for 21 d to induce rooting,subsequently transplanted into potting substrate for continued conservation,with their post-transplant growth recovery systematically monitored.The results demonstrated that hydroponic treatment induced robust rooting in weak seedlings,with 78.5％of accessions(line)producing 4-18 roots per plant and 88.1％exhibiting an average root length of 0.6-3.1 cm.At 20 d post-transplantation,the overall survival rate recorded 82.2％.After five months,96％of the accessions(line)produced vine lengths longer than 30 cm,A significant increase was observed in basal branching number,while 91％attained stem diameters greater than 3.0 mm,confirming that the overall growth met the established criteria for successful rejuvenation.Virus elimination was subsequently applied to the hydroponically-rejuvenated accessions(line),achieving a success rate of 90.1％.Virus elimination significantly restored the tuberization capacity in conventional seedlings of non-tuberizing accessions.The results showed that 82.4％of accessions(line)with virus-free seedlings achieved a yield exceeding 1000 kg per mu,whereas only 27.5％of conventional seedlings from the same accession reached equivalent production levels.The hydroponic rejuvenation technique not only enhanced the survival capacity of weak germplasm seedlings but also secured a critical time window for subsequent virus elimination,thereby providing a reliable and viable approach for the rejuvenation and conservation of weak seedlings within sweet potato germplasm resources.关键词
种质资源保存/甘薯种质资源退化/水培技术/弱苗复壮Key words
conservation of germplasm resources/degradation of sweet potato germplasm resources/hydroponic technology/rejuvenation of weak seedlings引用本文复制引用
姚肖健,王章英,杨义伶,唐朝臣,植美现,姚祝芳..基于水培技术的甘薯种质弱苗复壮方法研究[J].植物遗传资源学报,2026,27(4):789-802,14.基金项目
国家科技资源共享服务平台项目(NCGRC-2025-39) （NCGRC-2025-39）
中央财政农业生产发展资金农业种质资源保护任务 （）
广州市科技计划项目(2025D04J0085)National Science and Technology Resource Sharing Service Platform Project(NCGRC-2025-39) （2025D04J0085）
Central Fiscal Funds for Agricultural Production Development-Agricultural Germplasm Resources Conservation （）
Guangzhou City Science and Technology Program Project(2025D04J0085) （2025D04J0085）
