中国中西医结合杂志2026，Vol.46Issue(4)：452-458,7.DOI:10.7661/j.cjim.20260119.002
苁蓉舒痉颗粒减轻线粒体损伤抑制帕金森病神经炎症
Congrong Shujing Granules Alleviate Mitochondrial Damage and Inhibit Neuroinflammation in Parkinson's Disease
摘要
Abstract
Objective To investigate the mechanism by which Congrong Shujing Granules(CSG)alleviate neuroinflammation in Parkinson's disease(PD)by regulating dynamin-related protein 1(Drp1)-mediated mitochondrial damage and the NOD-like receptor family pyrin domain-containing 3(NLRP3)inflammasome pathway.Methods Forty-eight SPF-grade healthy male C57BL/6J mice were randomly divided into the control group,model group,CSG group and Madopar group using a random number table,with 12 mice in each group.A PD mouse model was induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP).The mice were treated with CSG or Madopar for 4 weeks.Motor function,dopaminergic neuronal integrity,mitochondrial function,and inflammatory responses were systematically evaluated using behavioral tests,immunohistochemistry,immunofluorescence,transmission electron microscopy,flow cytometry,Western Blot,and ELISA.Results Compared with the control group,the model group exhibited significant motor dysfunction,decreased tyrosine hydroxylase(TH)expression in the substantia nigra,increased ionized calcium-binding adaptor molecule 1(IBA1)expression,marked mitochondrial structural damage,and elevated reactive oxygen species(ROS)levels.In addition,the expression of phosphoglycerate mutase family member 5(PGAM5)and Drp1 were upregulated,the p-Ser637 Drp1/Drp1 ratio was decreased,and the levels of NLRP3 inflammasome-related proteins[including apoptosis-associated speck-like protein containing a CARD(ASC)and Caspase-1],as well as inflammatory cytokines(IL-1 β,IL-6,TNF-α),and iNOS were increased(P＜0.05).Compared with the model group,CSG improved motor function,increased TH expression,reduced IBA1 expression,attenuated mitochondrial damage and ROS accumulation,downregulated PGAM5 and Drp1 expression,increased the p-Ser637 Drp1/Drp1 ratio,and decreased NLRP3 inflammasome-related proteins and inflammatory cytokines levels(P＜0.05).Conclusion CSG exerts anti-neuroinflammatory and dopaminergic neuroprotective effects in PD by regulating Drp1-mediated mitochondrial dynamics,reducing ROS generation,and subsequently inhibiting NLRP3 inflammasome activation.关键词
帕金森病/苁蓉舒痉颗粒/线粒体损伤/神经炎症/NOD样受体热蛋白结构域相关蛋白3/中药复方Key words
Parkinson's disease/Congrong Shujing Granules/mitochondrial damage/neuroinflammation/NOD-like receptor family pyrin domain-containing 3/Chinese herbal compound引用本文复制引用
夏金言,成慧灵,张楚天,李珣,郑美玲,陈诗雅,李茜羽,蔡晶..苁蓉舒痉颗粒减轻线粒体损伤抑制帕金森病神经炎症[J].中国中西医结合杂志,2026,46(4):452-458,7.基金项目
国家自然科学基金面上项目(No.82074507,No.82474605) （No.82074507,No.82474605）
