中国中西医结合杂志2026，Vol.46Issue(4)：459-464,6.DOI:10.7661/j.cjim.20251118.029
肾元颗粒调节Klotho/NF-κB通路改善慢性肾脏病大鼠认知功能
Shenyuan Granules Improve Cognitive Function in Chronic Kidney Disease Rats by Regulating Klotho/NF-κB Pathway
摘要
Abstract
Objective To investigate the effects of Shenyuan Granules(SYG)on inflammatory response and cognitive function in rats with chronic kidney disease(CKD)based on the Klotho(KI)/nuclear factor-κB(NF-κB)signalling pathway.Methods A CKD model was established in rats via 5/6 nephrectomy.The rats were divided into sham-operated group,model group,SYG group,and positive drug group,with 6 rats in each group.The SYG group was administered SYG(6.0 g/kg)by gavage once daily,while the positive drug group received donepezil hydrochloride(10 mg/kg).The sham-operated and model groups were given an equal volume of saline.Cognitive function was assessed using the Morris water maze test.Serum levels of urea nitrogen(BUN)and creatinine(Cr)were measured biochemically.Serum concentrations of TNF-α and IL-1β were detected by ELISA.Protein expression of KI and NF-κB in the kidney and brain was determined by Western Blot.Pathological changes in the hippocampus and kidney were observed under light microscopy.Results Compared with the sham-operated group,the model group exhibited prolonged escape latency(P＜0.01),reduced number of platform crossings and duration in the target quadrant(P＜0.0001),elevated serum BUN and Cr levels(P＜0.0001),increased TNF-α and IL-1β concentrations(P＜0.0001),decreased KI protein expression in kidney and brain tissues(P＜0.0001),and increased NF-κB protein expression(P＜0.0001).Compared with the model group,both the SYG and positive drug groups showed shortened escape latency(P＜0.001),increased platform crossings(P＜0.05),prolonged platform stay(P＜0.01),decreased BUN and Cr(P＜0.05,P＜0.01,P＜0.0001),reduced TNF-α and IL-1β concentrations(P＜0.0001),upregulated KI protein expression(P＜0.001,P＜0.0001),and downregulated NF-κB protein expression(P＜0.001,P＜0.0001))in kidney and brain.No significant pathological changes were observed in the sham-operated group.The model group showed marked pathological changes in the hippocampus and kidney,which were ameliorated in the SYG and positive drug groups.Conclusions SYG may alleviate inflammation and improve cognitive function in CKD rats,potentially by regulating the KI/NF-κB pathway.关键词
慢性肾脏病/肾元颗粒/克老素/炎症/认知/中药复方Key words
chronic kidney disease/Shenyuan Granules/Klotho/inflammation/cognition/Chinese herbal compound引用本文复制引用
王长江,谢宇娟,吴文静,林腊梅,吴成态,邹新蓉,王小琴..肾元颗粒调节Klotho/NF-κB通路改善慢性肾脏病大鼠认知功能[J].中国中西医结合杂志,2026,46(4):459-464,6.基金项目
国家自然科学基金资助项目(No.82374384,No.82074395) （No.82374384,No.82074395）
国家自然科学基金青年基金项目(No.82304910) （No.82304910）
湖北省自然科学基金创新发展联合基金项目(No.2022CFD136) （No.2022CFD136）
湖北省自然科学基金一般面上项目(No.2024AFB925) （No.2024AFB925）
湖北省中医院院级课题(No.2021YJKT-18) （No.2021YJKT-18）
第七批全国老中医药专家学术经验继承工作项目(No.国中医药人教函[2022]76号) （No.国中医药人教函[2022]76号）
湖北省中医药管理局中医药科研项目(No.ZY2025M013) （No.ZY2025M013）
