中药新药与临床药理2026，Vol.37Issue(4)：607-622,16.DOI:10.19378/j.issn.1003-9783.2026.04.004
益气解毒方通过miR-3127-5p负调控STAT2抑制鼻咽癌细胞增殖、迁移和侵袭
Yiqi Jiedu Formula Inhibits Proliferation,Migration,and Invasion of Nasopharyngeal Carcinoma Cells Through miR-3127-5p-Mediated Negative Regulation of STAT2
摘要
Abstract
s:Objective To investigate the molecular mechanism by which Yiqi Jiedu Formula suppresses proliferation,migration,and invasion of nasopharyngeal carcinoma cells via miR-3127-5p-mediated negative regulation of signal transducer and activator of transcription 2(STAT2).Methods Animal experiments:A nasopharyngeal carcinoma xenograft model was established in nude mice and divided into solvent control group,Yiqi Jiedu Formula group,and 5-fluorouracil group,with 5 mice per group.Hematoxylin-eosin staining was used to observe histopathological morphology of nasopharyngeal carcinoma tissues.Immunohistochemistry and Simple Western assays were employed to assess the expression levels of proliferation-and metastasis-related proteins in tumor tissues.Cell experiment 1:Nasopharyngeal carcinoma cells overexpressing miR-3127-5p were treated with Yiqi Jiedu Formula(0.5 mg·mL-1)and divided into oe-miR-NC group,oe-miR-NC+Yiqi Jiedu Formula group,oe-miR-3127-5p group,and oe-miR-3127-5p+Yiqi Jiedu Formula group.Real-time cellular analysis(RTCA)was performed to evaluate cell proliferation.Wound healing assay and Matrigel invasion chamber assay were used to assess changes in cell migration and invasion abilities.Western Blot was conducted to determine protein expression levels.Cell experiment 2:Cells overexpressing both miR-3127-5p and STAT2 were treated with Yiqi Jiedu Formula(0.5 mg·mL-1)and divided into oe-miR-NC+oe-NC group,oe-miR-NC+oe-NC+Yiqi Jiedu Formula group,oe-miR-3127-5p+oe-NC group,oe-miR-3127-5p+oe-NC+Yiqi Jiedu Formula group,oe-miR-3127-5p+oe-STAT2 group,and oe-miR-3127-5p+oe-STAT2+Yiqi Jiedu Formula group.RTCA was used to measure cell proliferation,while wound healing and Matrigel invasion assays were employed to evaluate migration and invasion capacities.Results Hematoxylin-eosin staining revealed that the Yiqi Jiedu Formula group exhibited disorganized nasopharyngeal carcinoma tissue structure,accompanied by necrosis and vacuolation.Yiqi Jiedu Formula also reduced the expression levels of XIAP,PCNA,survivin,ID1,occludin,vimentin,claudin1,and F-actin in nasopharyngeal carcinoma tissues(P＜0.05,P＜0.01).Compared with the oe-miR-NC group,Yiqi Jiedu Formula decreased the relative proliferation rate,migration rate,and invasion rate of nasopharyngeal carcinoma cells(P＜0.05,P＜0.01),and reduced the expression levels of XIAP,PCNA,survivin,vimentin,F-actin,and ANXA1(P＜0.05,P＜0.01).Compared with the oe-miR-NC+Yiqi Jiedu Formula group,the miR-3127-5p+Yiqi Jiedu Formula group showed significantly lower relative proliferation,migration,and invasion rates(P＜0.05,P＜0.01),along with decreased expression of XIAP,PCNA,survivin,vimentin,F-actin,and ANXA1 in nasopharyngeal carcinoma cells(P＜0.05,P＜0.01).Factorial design analysis indicated an interactive effect between Yiqi Jiedu Formula and miR-3127-5p in inhibiting proliferation,migration,and invasion of nasopharyngeal carcinoma cells.Intervention with Yiqi Jiedu Formula in cells overexpressing both miR-3127-5p and STAT2 demonstrated that,compared with the oe-miR-3127-5p+oe-NC+Yiqi Jiedu Formula group,the oe-miR-3127-5p+oe-STAT2+Yiqi Jiedu Formula group exhibited a statistically significant increase in relative proliferation rate at 24 h in S18 cells(P＜0.05),while no statistical significance was observed at 36 h and 48 h(P＞0.05).In 5-8F cells,the increase in relative proliferation rate at 24 h,36 h,and 48 h was not statistically significant(P＞0.05),but the relative migration and invasion rates were significantly elevated(P＜0.01).Conclusion Yiqi Jiedu Formula inhibits proliferation,migration,and invasion of nasopharyngeal carcinoma cells through miR-3127-5p-mediated negative regulation of STAT2.关键词
益气解毒方/miR-3127-5p/STAT2/鼻咽癌/人鼻咽癌细胞 5-8F/人鼻咽癌细胞 S18/增殖/迁移/侵袭Key words
Yiqi Jiedu Formula/miR-3127-5p/STAT2/nasopharyngeal carcinoma/human nasopharyngeal carcinoma cell line 5-8F/human nasopharyngeal carcinoma cell line S18/proliferation/migration/invasion分类
医药卫生引用本文复制引用
刘洁,张文青,史红健,范婧莹,杨涵中,何迎春..益气解毒方通过miR-3127-5p负调控STAT2抑制鼻咽癌细胞增殖、迁移和侵袭[J].中药新药与临床药理,2026,37(4):607-622,16.基金项目
湖南省自然科学基金项目(2023JJ30449,2024JJ6343) （2023JJ30449,2024JJ6343）
湖南省教育厅资助项目(23A0298,23B0361) （23A0298,23B0361）
湖南省卫生健康高层次人才重大专项(R2023111) （R2023111）
湖南省卫健委项目(NO.D202307017740) （NO.D202307017740）
长沙市科技局指导项目(kzd22008) （kzd22008）
湖南中医药大学校级课题(Z2023XJYB08). （Z2023XJYB08）
