中药新药与临床药理2026，Vol.37Issue(4)：637-643,7.DOI:10.19378/j.issn.1003-9783.2026.04.006
基于网络药理学和分子对接技术探讨党参治疗糖尿病性骨质疏松症的作用机制
Exploring the Mechanism of Codonopsis Radix in Treating Diabetic Osteoporosis Based on Network Pharmacology and Molecular Docking Technology
摘要
Abstract
Objective To investigate the mechanism of Codonopsis Radix in treating diabetic osteoporosis(DOP)using network pharmacology and molecular docking technology.Methods The active components of Codonopsis Radix and their corresponding targets were obtained through literature retrieval and the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP).Targets related to DOP were screened using the GeneCards and OMIM databases.The intersection targets between the active component targets of Codonopsis Radix and DOP-related targets were identified.A protein-protein interaction(PPI)network and a"Codonopsis Radix-component-target"network diagram were constructed using the STRING database and Cytoscape 3.8.0 software,respectively,to screen out the core targets and active components of Codonopsis Radix for DOP treatment.The DAVID database was used for Gene Ontology(GO)functional and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses of the intersection targets.Molecular docking technology was employed to evaluate the binding activity between the core targets for DOP treatment and the core active components of Codonopsis Radix.Results A total of 39 active components related to Codonopsis Radix and 610 corresponding targets were screened.The GeneCards and OMIM databases yielded 1 715 DOP-related targets.The intersection of these two sets identified 210 potential targets for Codonopsis Radix in treating DOP.The core targets for Codonopsis Radix in treating DOP included non-receptor tyrosine kinase(SRC),signal transducer and activator of transcription 3(STAT3),phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha(PIK3CA),phosphinositide-3-kinase regulatory subunit 1(PIK3R1),and heat shock protein 90α family class A member 1(HSP90AA1).The core active components included 7-methoxy-2-methyl isoflavone,luteolin,apigenin,9,10-dihydroxy-12-octadecenoic acid,and 11-hydroxyrankinidine.KEGG pathway enrichment analysis revealed that the mechanisms of Codonopsis Radix in treating DOP are related to signaling pathways such as the PI3K/Akt,HIF-1,and AGE-RAGE signaling pathways in diabetic complications.Molecular docking demonstrated that the five core active components of Codonopsis Radix(7-methoxy-2-methyl isoflavone,luteolin,apigenin,9,10-dihydroxy-12-octadecenoic acid,and 11-hydroxyrankinidine)exhibited favorable binding activities(all<-5 kcal·mol-1)with core targets like SRC,STAT3,PIK3CA,and PIK3R1.Conclusion Codonopsis Radix exerts therapeutic effects on diabetic osteoporosis through multiple components,targets,and pathways.Its mechanism of action may be associated with signaling pathways such as PI3K/Akt,HIF-1,and the AGE-RAGE signaling pathway in diabetic complications.关键词
党参/糖尿病性骨质疏松症/网络药理学/分子对接/作用机制Key words
Codonopsis Radix/diabetic osteoporosis/network pharmacology/molecular docking/mechanism of action分类
医药卫生引用本文复制引用
王丽,吴培阳,谢保城,徐永祥..基于网络药理学和分子对接技术探讨党参治疗糖尿病性骨质疏松症的作用机制[J].中药新药与临床药理,2026,37(4):637-643,7.基金项目
广东省医学科研基金项目(A2024138,B2025580) （A2024138,B2025580）
广东省医院药学研究基金项目(2025A01002). （2025A01002）
