中药药理与临床2026，Vol.42Issue(3)：10-17,8.
基于UHPLC-MS/MS代谢组学探讨金匮肾气丸对慢性肾病肾间质纤维化的改善作用及其机制研究
Effects and Mechanisms of Jingui Shenqi Pills in Ameliorating Renal Interstitial Fibrosis in Chronic Kidney Disease Based on UHPLC-MS/MS Metabolomics
摘要
Abstract
Objective:To investigate the pharmacodynamic effects of Jinkui Shenqi(金匮肾气丸)Pills on renal interstitial fibrosis(RIF)in chronic kidney disease(CKD)and to explore the potential mechanisms using UHPLC-MS/MS metabolomics.Methods:SD rats were randomly divided into a normal control group,a model control group,a Losar-tan group,and Jinkui Shenqi Pills groups at doses of 0.6,1.2,and 2.4 g/kg.CKD was induced by oral administration of adenine(200 mg/kg)for 3 weeks,followed by 7 weeks of drug treatment.Prior to sampling,rats were housed in meta-bolic cages for 24 hours to collect urine.One hour after the last administration,blood samples were collected to measure serum urea nitrogen(BUN)and creatinine(Crea),and urinary Crea was measured to calculate the creatinine clearance rate(CCr).Kidney pathology was assessed using hematoxylin-eosin(HE)and Masson's trichrome staining.Endogenous serum metabolites were analyzed by ultrahigh-performance liquid chromatography coupled with tandem mass spectrometry(UHPLC-MS/MS).Results:Compared with the normal control group,the model group showed significantly increased serum BUN and Crea levels(P<0.01)and significantly decreased CCr(P<0.01).Compared with the model group,Jinkui Shenqi Pills significantly reduced serum BUN and Crea levels(P<0.05 or P<0.01)and markedly increased CCr(P<0.01).Histopathological analysis indicated that Jinkui Shenqi Pills ameliorated adenine-induced renal lesions,in-cluding glomerular atrophy,renal tubular dilation,sparse cellular arrangement,loss of brush borders,inflammatory cell in-filtration,and collagen fiber deposition in the renal interstitium.Metabolomic analysis revealed that Jinkui Shenqi Pills modulated 132 differential metabolites and enriched 63 metabolic pathways,among which 9 were identified as key path-ways.Conclusion:Jinkui Shenqi Pills can inhibit adenine-induced renal interstitial fibrosis,and its mechanism may be related to the regulation of amino acid metabolism,lipid metabolism,and energy metabolism.关键词
金匮肾气丸/慢性肾病/肾间质纤维化/代谢组学/肾功能Key words
Jinkui Shenqi Pills/Chronic kidney disease/Renal interstitial fibrosis/Metabolomics/Renal function引用本文复制引用
李安,陈云贵,谢高宇,晋海军,梁建东,杨芳芳,蔡琨,刘琴,王丽颖..基于UHPLC-MS/MS代谢组学探讨金匮肾气丸对慢性肾病肾间质纤维化的改善作用及其机制研究[J].中药药理与临床,2026,42(3):10-17,8.基金项目
贵州中医药大学学术新苗项目(贵科合学术新苗[2023]-29号) （贵科合学术新苗[2023]-29号）
贵州省科协"新质黔沿"引领项目(编号:2025XZQYXM-02-04) （编号:2025XZQYXM-02-04）
贵州省科技创新人才团队(黔科合平台人才[2020]5010) （黔科合平台人才[2020]5010）
贵州省发改委工程研究中心建设项目(黔发改高技[2020]896号) （黔发改高技[2020]896号）
贵州省苗医药全省重点实验室(黔科合平台ZSYS[2025]018). （黔科合平台ZSYS[2025]018）
