现代牧业2026，Vol.10Issue(1)：16-21,6.
双酶酶解联合乳酸菌发酵黑水虻粗提多肽体外抗氧化活性比较分析
Comparative analysis of in vitro antioxidant activity of crude BSF peptides prepared by dual-enzymatic hydrolysis coupled with Lactic Acid Bacteria Fermentation
摘要
Abstract
To evaluate the in vitro antioxidant activity of polypeptides prepared from black soldier fly(Hermetia illucens)processed by enzymatic hydrolysis alone or by enzymatic hydrolysis combined with fermentation using three lactic acid bacteria,four treatments were established with three replicates each:a dual-enzyme(alkaline protease and flavourzyme)hydrolysis group(control),group 1(hydrolysis combined with Lactobacillus plantarum fermentation),group 2(hydrolysis combined with Enterococcus faecalis fermentation),and group 3(hydrolysis combined with Lactobacillus acidophilus fermentation).After enzymatic hydrolysis and fermentation,the products were subjected to centrifugation,molecular-weight cut-off ultrafiltration,and freeze drying to obtain crude polypeptide extracts.Vitamin C(Vc)was used as a reference,and the antioxidant activities of the four crude polypeptide extracts were assessed in vitro.The results showed that,compared with the control,crude polypeptides from all three enzyme-microbe co-fermentation groups exhibited significantly higher total reducing power,hydroxyl radical scavenging activity,superoxide anion scavenging activity,and DPPH radical scavenging activity(P<0.05).Notably,when the crude polypeptide concentration reached 25 mg/mL,the total reducing power of the extracts from group 1 and group 3 was comparable to that of 1 mg/mL Vc.Moreover,at most tested concentrations,crude polypeptides from group 1 and group 3 showed significantly greater total reducing power and radical scavenging activities than those from group 2(P<0.05).These findings provide a basis for developing black soldier fly-derived functional feed additives.关键词
黑水虻/粗提多肽/抗氧化活性/发酵Key words
Black soldier fly(BSF)/crude peptides/antioxidant activity/fermentation分类
农业科技引用本文复制引用
姬向波,郭建来,薛楠楠,张蓓,许慧芳,王清凤..双酶酶解联合乳酸菌发酵黑水虻粗提多肽体外抗氧化活性比较分析[J].现代牧业,2026,10(1):16-21,6.基金项目
河南省科技攻关项目(232102110090) （232102110090）
河南省科技攻关项目(22102770390). （22102770390）
