首页|期刊导航|Journal of Integrative Agriculture|A rapid and visual detection method for porcine deltacoronavirus with single-copy sensitivity based on the CRISPR/Cas12a assay

A rapid and visual detection method for porcine deltacoronavirus with single-copy sensitivity based on the CRISPR/Cas12a assay

Zezhao Cao Zi Li Kaikai Jin Zanheng Huang Ruijie Hu Junchao Shi Huabo Yu Gaili Wang Wenqi He Hualei Wang Yungang Lan Haili Zhang

Journal of Integrative Agriculture2026，Vol.25Issue(4)：P.1736-1739,4.

Journal of Integrative Agriculture2026，Vol.25Issue(4)：P.1736-1739,4.DOI:10.1016/j.jia.2025.07.030

A rapid and visual detection method for porcine deltacoronavirus with single-copy sensitivity based on the CRISPR/Cas12a assay

Zezhao Cao ¹Zi Li ¹Kaikai Jin ¹Zanheng Huang ¹Ruijie Hu ¹Junchao Shi ¹Huabo Yu ¹Gaili Wang ²Wenqi He ¹Hualei Wang ¹Yungang Lan ¹Haili Zhang¹

作者信息

1. State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases,Key Laboratory for Zoonosis Research of the Ministry of Education,Institute of Zoonosis,College of Veterinary Medicine,Jilin University,Changchun 130062,China
2. Jilin Academy of Animal Husbandry and Veterinary Medicine,Changchun 130062,China
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摘要

关键词

CRISPR Cas/aerosol contamination rt raa crispr cas/pdco v/Porcine deltacoronavirus/Visual detection/False positives/Rapid detection/RT RAA

分类

农业科技

引用本文复制引用

Zezhao Cao,Zi Li,Kaikai Jin,Zanheng Huang,Ruijie Hu,Junchao Shi,Huabo Yu,Gaili Wang,Wenqi He,Hualei Wang,Yungang Lan,Haili Zhang..A rapid and visual detection method for porcine deltacoronavirus with single-copy sensitivity based on the CRISPR/Cas12a assay[J].Journal of Integrative Agriculture,2026,25(4):P.1736-1739,4.

基金项目

financially supported by the National Key Research and Development Program of China(2021YFF0703600)。（2021YFF0703600）

Journal of Integrative Agriculture

ISSN：2095-3119

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