安徽医科大学学报2026，Vol.61Issue(3)：439-447,9.DOI:10.19405/j.cnki.issn1000-1492.2026.03.008
BMP3抑制大鼠佐剂诱导性关节炎的炎症反应
BMP3 inhibits the inflammatory response in rat adjuvant-induced arthritis
摘要
Abstract
Objective To investigate the effect of bone morphogenetic protein 3(BMP3)on the expression of in-flammatory factors and joint damage in adjuvant arthritis(AA)induced by Freund's complete adjuvant(FCA)in rats.Methods The AIA model was established in SD rats by intradermal injection of FCA into the toes of the left hind limb,and BMP3 overexpressing adenovirus(Ad-BMP3)or control adenovirus(Ad-NC)was injected in situ into the knee joint cavity on day 8 after modeling.Subsequently,HE staining was used to observe the histopatho-logical changes in the synovium,immunohistochemistry was used to detect the expression of BMP3 in the synovium,and ELISA was used to analyze the expression levels of IL-6,IL-1β and TNF-α in the serum.Primary fibroblast-like synoviocytes(FLS)were isolated from AIA rats,the expression of BMP3 in FLS was knocked down or overexpressed,and Western blot and qRT-PCR were used to detect the expression levels of BMP3 and inflamma-tory factors in FLS.Results HE staining confirmed the successful establishment of the AIA model.Compared with normal rats,AIA rats showed decreased BMP3 expression in synovial tissue.Knockdown of BMP3 promoted the protein expression of inflammatory factors(IL-6,IL-1β,IL-17A,TNF-α)and the mRNA expression of chemo-kines[C-C motif chemokine ligand 2(CCL2),C-C motif chemokine ligand 3(CCL3),Vascular Cell Adhesion Molecule-1(VCAM-1)]in FLS.In contrast,overexpression of BMP3 suppressed the expression of these inflamma-tory factors and chemokines.Intra-articular injection of BMP3-overexpressing adenovirus in AIA rats upregulated BMP3 expression in synovial tissue and inhibited synovial inflammation and bone erosion.Conclusion BMP3 sup-presses the production of inflammatory factors and chemokines in FLS,thereby alleviating synovial hyperplasia and bone erosion in arthritis.关键词
BMP3/佐剂性关节炎/成纤维样滑膜细胞/炎症因子/AIA/腺病毒Key words
BMP3/adjuvant-induced arthritis/fibroblast-like synoviocytes/inflammatory factors/AIA/adeno-virus分类
医药卫生引用本文复制引用
孙丹彤,李小枫,李俊,宋标..BMP3抑制大鼠佐剂诱导性关节炎的炎症反应[J].安徽医科大学学报,2026,61(3):439-447,9.基金项目
国家自然科学基金项目(编号:82002269) （编号:82002269）
安徽医科大学校科研基金项目(编号:2022xkj141) （编号:2022xkj141）
安徽医科大学炎症免疫性疾病安徽省实验室开放课题项目(编号:IMMDL202102) National Natural Science Foundation of China(No.82002269) （编号:IMMDL202102）
Scientific Research Project of Anhui Medical University(No.2022xkj141) （No.2022xkj141）
Open Project of Anhui Provincial Laboratory of Inflammatory and Immune Diseases,Anhui Medical University(No.IMMDL202102) （No.IMMDL202102）
