安徽医科大学学报2026，Vol.61Issue(3)：455-461,7.DOI:10.19405/j.cnki.issn1000-1492.2026.03.010
雄激素对去势雄性小鼠认知功能的影响
Effects of androgens on cognitive function in castration male mice
摘要
Abstract
Objective To establish a castrated male mouse model and to preliminarily investigate the effects of tes-tosterone replacement therapy(TRT)on behavior,serum indices,and histopathological changes in castrated mice,as well as to explore the role of androgens in cognitive function.Methods Forty 6-month-old male C57/BL6J mice were randomly divided into sham operation group,castration group,testosterone propionate(0.5,1.0 mg/kg)treated group,with 10 mice in each group.Following castration and subcutaneous administration of testosterone propionate at different doses(0.5 and 1.0 mg/kg)for TRT,learning and memory abilities were as-sessed using the Morris water maze(MWM)test and the passive avoidance test.Serum testosterone and serum brain-derived neurotrophic factor(BDNF)levels were measured by ELISA,and histopathological changes in the hippocampus were examined using hematoxylin-eosin(HE)staining.Results Routine observations:there were no statistically significant differences in body weight among groups at any time point.MWM test:compared with castration group,sham operation group and testosterone propionate-treated groups(0.5,1.0 mg/kg)showed sig-nificantly reduced escape latency on days 4 and 5(P<0.05),while the number of platform crossings and the time spent in the target quadrant significantly increased(P<0.05).Passive avoidance test:the number of passive avoid-ance errors significantly decreased in sham operation group and testosterone propionate(1.0 mg/kg)-treated group(P<0.05),and the passive avoidance latency was significantly prolonged in sham-operated group and testosterone propionate-treated groups(0.5,1.0 mg/kg)(P<0.05).Serum testosterone and serum BDNF assays:serum tes-tosterone levels and serum BDNF concentrations significantly increased in sham operation group and testosterone propionate-treated groups(0.5,1.0 mg/kg)(P<0.01).HE staining:compared with sham operation group,neu-ronal density in all hippocampal subregions was slightly reduced in castration group;in the testosterone propionate(0.5 mg/kg)-treated group,neuronal arrangement in the CA1 and CA3 regions was improved and apoptotic cells were reduced compared with castration group;in testosterone propionate(1.0 mg/kg)-treated group,the pyrami-dal cell layer in the CA3 region was more compactly arranged,with fewer apoptotic cells than in castration group.Conclusion TRT improves learning and memory performance in castration male mice,potentially through modu-lation of hippocampal BDNF signaling pathways.关键词
雄激素/丙酸睾酮/去势/TRT/行为学实验/脑源性神经营养因子/认知Key words
androgen/testosterone propionate/castration/TRT/behavioral tests/brain-derived neurotrophic factor/cognition分类
医药卫生引用本文复制引用
张雅琪,惠灿灿,任芳,许敏,姜子龙,邓大同..雄激素对去势雄性小鼠认知功能的影响[J].安徽医科大学学报,2026,61(3):455-461,7.基金项目
安徽省高校自然科学研究重点项目(编号:2022AH051141) Natural Science Research Project of Anhui Educational Committee(No.2022AH051141) （编号:2022AH051141）
