安徽医科大学学报2026，Vol.61Issue(4)：606-612,7.DOI:10.19405/j.cnki.issn1000-1492.2026.04.003
RUNX3通过调控FAP对小鼠肺原代成纤维细胞增殖的影响
RUNX3 regulates FAP to influence the proliferation of mouse lung primary fibroblasts
摘要
Abstract
Objective To investigate the role of runt-related transcription factor 3(RUNX3)in transforming growth factor-β1(TGF-β1)-induced activation of mouse primary pulmonary fibroblasts(PFs),and its effects on fi-broblast activation protein(FAP)expression,cell proliferation,and collagen synthesis.Methods PFs were iso-lated from C57BL/6 mice and cultured.A RUNX3 knockdown model was established using small interfering RNA(siRNA).Cells were assigned to the control group(Control),TGF-β1-treated group(TGF-β1),negative control group(TGF-β1+siRNA-NC),and RUNX3-silenced group(TGF-β1+si-RUNX3).In addition,a RUNX3 overex-pression rescue experiment was performed based on TGF-β1 stimulation.Protein and mRNA levels of RUNX3,FAP,and typeⅠcollagen(COL1A1)were measured by Western blot and reverse transcription quantitative real-time PCR(RT-qPCR).Cell proliferation was assessed using CCK-8 and EdU assays.Co-expression of COL1A1 and FAP was examined by double immunofluorescence staining.Results Compared with the Control group,RUNX3,FAP,and COL1A1 expression levels were upregulated in PFs in the TGF-β1 group(P<0.01).The CCK-8 assay showed that the absorbance value was reduced in the RUNX3 knockdown group compared with the negative control group(P<0.01).Consistently,the EdU assay demonstrated a lower proportion of EdU-positive cells in the RUNX3 knockdown group than in the negative control group(P<0.01).Immunofluorescence double staining re-vealed decreased fluorescence intensities of COL1A1 and FAP in the RUNX3 knockdown group relative to the nega-tive control.Under RUNX3 overexpression conditions,these fluorescence signals exhibited a partial rebound(P<0.01).Conclusion RUNX3 in TGF-β1-induced PFs may promote cell proliferation and collagen synthesis by positively regulating FAP expression.Targeting the RUNX3/FAP axis may represent a potential therapeutic strat-egy for pulmonary fibrosis.关键词
RUNX3/肺成纤维细胞/FAP/细胞增殖/肺纤维化Key words
RUNX3/primary pulmonary fibroblasts/fibroblast activation protein/proliferation/pulmonary fibro-sis分类
医药卫生引用本文复制引用
尤峻柏,汪先晨,凌辉,范家好,陈齐,陶辉,沙纪名..RUNX3通过调控FAP对小鼠肺原代成纤维细胞增殖的影响[J].安徽医科大学学报,2026,61(4):606-612,7.基金项目
安徽省高校自然科学研究项目(编号:2023AH040376) Natural Science Research Project of Anhui Educational Committee(No.2023AH040376) （编号:2023AH040376）
