安徽医科大学学报2026，Vol.61Issue(4)：628-635,8.DOI:10.19405/j.cnki.issn1000-1492.2026.04.006
裂果薯皂苷Ⅰ体外抑制鼻咽癌细胞HONE-1作用及其机制
The effect and mechanism of Saponin Ⅰ of Schizocapsa plantaginea Hance on nasopharyngeal carcinoma cell line HONE-1 in vitro
摘要
Abstract
Objective To explore the inhibitory effect and related molecular mechanisms of Saponin of Schizo-capsa plantaginea HanceⅠ(SSPHⅠ)on human nasopharyngeal carcinoma HONE-1 cells.Methods The effect of SSPHⅠ on HONE-1 cell viability was detected using the CCK-8 assay.Its inhibitory effect on cell proliferation was evaluated through a colony formation assay.Changes in cell invasion ability were analyzed using the Transwell assay.Intracellular reactive oxygen species(ROS)levels were measured using the DHE fluorescent probe.The ex-tent of intracellular content release was reflected by the LDH release assay.The rate of cell pyroptosis was detected using the Annexin-V/PI double staining method.Changes in the expression of proteins related to the classical py-roptosis pathway were examined by Western Blot.Results CCK-8 assay showed that treatment with SSPHⅠ for 24 hours reduced HONE-1 cell viability in a concentration-dependent manner,with an IC50 value of 3.383 μmol/L.In the colony formation assay,the number of HONE-1 cell colonies gradually decreased with increasing concentra-tions of SSPHⅠ(P<0.01).The Transwell assay revealed that the number of cells migrating through the chamber was reduced following SSPHⅠ treatment(P<0.01).DHE fluorescence probe detection indicated that intracellu-lar ROS fluorescence intensity increased after SSPHⅠ treatment(P<0.001).The LDH release assay showed that LDH activity in the cell supernatant increased with higher concentrations of SSPHⅠ(P<0.001).Annexin-V/PI double staining demonstrated that the proportion of Annexin-V/PI-positive cells increased after SSPHⅠ treatment(P<0.001).Western blot analysis showed that,compared with the control group,the protein expression levels of cleaved-Caspase-1 and GSDMD-N-terminal were upregulated in SSPH Ⅰ-treated cells(P<0.05),and NLRP3 protein expression levels also increased(P<0.05).ELISA results showed that the levels of IL-1β and IL-18 in the cells increased with higher concentrations of SSPHⅠ(P<0.05).Conclusion SSPHⅠ can induce pyroptosis in nasopharyngeal carcinoma HONE-1 cells by regulating the ROS/NLRP3/Caspase-1 signaling axis,thereby exert-ing an anti-nasopharyngeal carcinoma effect.This suggests that SSPHⅠ may serve as a potential therapeutic agent for nasopharyngeal carcinoma.关键词
SSPHⅠ/鼻咽癌/活性氧/NLRP3/焦亡/皂苷Key words
SSPHⅠ/nasopharyngeal carcinoma/reactive oxygen species/NLRP3/pyroptpsis/saponin分类
医药卫生引用本文复制引用
郭心怡,梁梓樱,王金妮,丁小莲,王燕雪,梁钢..裂果薯皂苷Ⅰ体外抑制鼻咽癌细胞HONE-1作用及其机制[J].安徽医科大学学报,2026,61(4):628-635,8.基金项目
国家自然科学基金项目(编号:82360792) （编号:82360792）
广西生物靶向诊治研究重点实验室开放课题项目(编 号:GXSWBX 202402) National Natural Science Foundation of China(No.82360792) （编 号:GXSWBX 202402）
Open Project of Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research(No.GXSWBX202402) （No.GXSWBX202402）
