安徽医科大学学报2026，Vol.61Issue(4)：653-661,9.DOI:10.19405/j.cnki.issn1000-1492.2026.04.009
AMPK活化在红景天苷改善动脉粥样硬化小鼠模型内皮祖细胞功能中的作用
Salidroside exerts cytoprotective effects on bone endothelial progenitor cells via the AMPK pathway in atherosclerotic mouse model
摘要
Abstract
Objective To investigate the effects of salidroside(SAL)on the impaired bioactivity of endothelial progenitor cells(EPCs)in atherosclerotic(As)mice and the potential mechanisms regarding AMP-activated pro-tein kinase(AMPK).Methods Atherosclerosis was induced in 8-week-old male ApoE-/-mice with high-fat diet.Intragastric administration of SAL was given to one mice group to investigate the effects of SAL on aortic plaque bur-den,plasma NO level,the migration and angiogenic capabilities of bone marrow-derived EPCs(BM-EPCs).The proliferation,migration and vasculogenic properties of EPCs isolated from As mice were investigated in vitro.AMPK-sh-RNA or the AMPK inhibitor Compound C was used to investigate the role of AMPK/Akt/eNOS pathway in the regulatory effects of SAL.Results Compared with As group,NO level was significantly elevated in SAL group.The sizes of atherosclerotic plaques at the aortic root were reduced with smaller lipid cores in SAL group compared with As group.Moreover,the migration and angiogenesis capacity of EPCs markedly decreased in As mice,while SAL treatment reversed these impairments.Incubation with SAL at concentrations of 20,40,and 80 μmol/L for 48 hours significantly promoted the proliferation,migration,and angiogenesis of EPCs.AMPK-sh-RNA transfection abrogated the 20 μmol/L SAL improvement in EPC biological activities.Western blot analysis further demonstrated that treatment with Compound C blocked the activation of AMPK/Akt/eNOS signaling pathway in-duced by SAL.Conclusion SAL upregulates the biological functions of EPCs through activating the AMPK/Akt/eNOS signaling pathway,thereby ameliorating EPC dysfunction during the pathological progression of atherosclero-sis.关键词
红景天苷/内皮祖细胞/腺苷酸激活蛋白激酶/动脉粥样硬化/内皮型一氧化氮合酶/AMPK/Akt/eNOS信号通路Key words
salidroside/endothelial progenitor cells/AMP-activated protein kinase/atherosclerosis/eNOS/AMPK/Akt/eNOS pathway分类
医药卫生引用本文复制引用
贾方,王梦非,费思凡,徐加怡,俞天虹,朱琳,周敏..AMPK活化在红景天苷改善动脉粥样硬化小鼠模型内皮祖细胞功能中的作用[J].安徽医科大学学报,2026,61(4):653-661,9.基金项目
国家自然科学基金项目(编号:81300220) （编号:81300220）
常州市卫健委重大科技项目(编号:ZD202212) （编号:ZD202212）
常州市卫生健康人才国内外研修资助项目(编号:GN2023006) （编号:GN2023006）
苏州工业园区东方华夏心血管健康研究院-天然调脂药物循证科研基金项目(编号:2023-CCA-NLD-431) （编号:2023-CCA-NLD-431）
常州市第四周期医学重点学科项目(编号:CZXK202202) National Natural Science Foundation of China(No.81300220) （编号:CZXK202202）
Major Science and Technology Program of Changzhou Health Commission(No.ZD202212) （No.ZD202212）
Domestic and International Training Support Pro-gram for Health Talent of Changzhou(No.GN2023006) （No.GN2023006）
Chinese Cardiovascular Association-Natural Lipid-lowering Drugs Fund(No.2023-CCA-NLD-431) （No.2023-CCA-NLD-431）
Changzhou Key Medical Discipline Fund(No.CZXK202202) （No.CZXK202202）
