北方园艺Issue(8)：73-81,9.DOI:10.11937/bfyy.20254053
双色万代兰种子形态及无菌萌发研究
Seed Morphology and Asymbiotic Germination of Vanda bicolor Griff.
摘要
Abstract
Taking V.bicolor mature seeds refrigerated at-25℃ for 135 days as test materials,the seed morphological characteristics and viability were investigated using stereomicroscopy and 0.5％TTC staining,respectively.An orthogonal experiment was conducted using MS as the basic medium to study the effects of different combinations of 6-BA,NAA,and coconut water(CW)under light and dark conditions for 180 days,to establish a suitable asymbiotic germination system,in order to provide a reference for the germplasm conservation and artificial breeding of the endangered Vanda bicolor.The results showed that the seeds of V.bicolor were brownish-black.The seed length and width were(0.301±0.019)mm and(0.098±0.009)mm,respectively,while the embryo length and width were(0.228±0.031)mm and(0.096±0.013)mm,respectively.The embryo occupied almost the entire seed coat cavity.TTC testing indicated that the seeds maintained a viability of 60.3％after long-term low-temperature storage.In the asymbiotic germination experiment,the optimal medium combination was MS+1.0 mg·L-1 6-BA+0 mg·L-1 NAA+100 mL·L-1 CW.Light conditions significantly improved the germination rate,while dark culture was conducive to protocorm expansion and differentiation.Range analysis revealed that the primary factors affecting germination were in the order of 6-BA＞CW＞NAA.In conclusion,6-BA and CW were the key factors promoting the seed germination of V.bicolor.A phased configuration of light and dark culture was beneficial for improving germination and protocorm growth.关键词
双色万代兰/种子形态/TTC/非共生萌发Key words
Vanda bicolor/seed morphology/TTC/asymbiotic germination分类
农业科技引用本文复制引用
周学青,陈学达,王永霞,章容杰,山晓丹,邢震..双色万代兰种子形态及无菌萌发研究[J].北方园艺,2026,(8):73-81,9.基金项目
林芝市林业和草原局重点资助项目(LZLCJ603324061) （LZLCJ603324061）
西藏自治区教育厅资助项目(XK2024-01,XK2024-04,YJSJD2025-6) （XK2024-01,XK2024-04,YJSJD2025-6）
西藏农牧学院林学博士点(一期)资助项目(533325001). （一期）
