川北医学院学报2026，Vol.41Issue(3)：278-283,6.DOI:10.3969/j.issn.1005-3697.2026.03.003
替泊沙林下调ABCB1介导AKT/mTOR通路调节LS1034细胞的迁移与凋亡
Tepoxalin modulates migration and apoptosis of LS1034 cells by down-regulating ABCB1-mediated AKT/mTOR pathway
摘要
Abstract
Objective:To explore the downregulation of ATP-binding cassette transporter B subfamily member 1(ABCB1)by Tepoxalin mediated AKT/mTOR pathway in regulating the migration and apoptosis of colorectal cancer(CRC)LS1034 cells and its possible mechanisms.Methods:Public databases(GEPIA and HPA)were used to analyze the expression level and prognosis of ABCB1 in CRC,CCK-8 was used to detect cell proliferation,RT-qPCR and Western blot were used for mRNA and protein detection,flow cytometry was used for apoptosis detection,and Transwell assay was used to measure cell migration.Results:ABCB1 was highly expressed in CRC tissues(P＜0.05)and was not correlated with prognosis(P＞0.05).Cell validation showed that ABCB1 was an upregulated gene in CRC.Tepoxalin inhibits ABCB1 in colorectal cancer cells and blocks the induction of proliferation,migration,and apoptosis in colorectal cancer cells.In addition,Western blot results showed that tepoxalin could inhibit AKT/mTOR signaling pathway and apoptotic signaling pathway expression.Conclusion:Tepoxalin inhibits proliferation and migration of CRC cells while inducing apoptosis by targeting ABCB1,which is mediated by ABCB1-mediated inhibition of AKT/mTOR signaling pathway.关键词
替泊沙林/ABCB1/结直肠癌/AKT/mTOR信号通路Key words
Tepoxalin/ABCB1/Colorectal cancer/AKT/mTOR signaling pathway分类
医药卫生引用本文复制引用
金丽雯,何治军,徐继..替泊沙林下调ABCB1介导AKT/mTOR通路调节LS1034细胞的迁移与凋亡[J].川北医学院学报,2026,41(3):278-283,6.基金项目
浦东新区卫生健康委员会卫生计生科研项目(PW2022A-24) （PW2022A-24）
