动物医学进展2026，Vol.47Issue(5)：89-95,7.
基于p72、CD2v、MGF360-13L和K196R基因的非洲猪瘟病毒实时荧光定量PCR检测方法的建立
Establishment of a Real-time Fluorescent Quantitative PCR for African Swine Fever Virus Based on p72、CD2v、MGF360-13L and K196R Genes
摘要
Abstract
African swine fever virus(ASFV)has caused huge losses to the global swine industry.Currently,strict biosecurity measures are the main defense,and accurate and rapid pathogen detection is crucial.However,existing nucleic acid testing methods have problems such as single diagnostic indicators and insufficient specificity.In this study,a quadruple RT-qPCR detection system was constructed using multiplex RT-qPCR technology to simultaneously detect four characteristic genes of ASFV:p72、CD2v、MGF360-13Land K196R,by designing primers and probes and optimizing reaction conditions.The experimental results showed that the lowest detection limit of this method was 225 copies/μL of recombinant plasmid,and the intra-and inter-batch variation coefficients were both below 2％,indicating that the established method has strong specificity,high sensitivity,and good reproducibility,and provides effective technical support for the accurate detection of ASFV.关键词
非洲猪瘟病毒/四重RT-qPCR/核酸检测Key words
African swine fever virus/Quadruplex RT-qPCR/Nucleic acid detection分类
农业科技引用本文复制引用
管大杉,杜付玉,贺安娜,王馨若,苏卯枝,杨鹏,张象涵,窦志强,宁蓬勃..基于p72、CD2v、MGF360-13L和K196R基因的非洲猪瘟病毒实时荧光定量PCR检测方法的建立[J].动物医学进展,2026,47(5):89-95,7.基金项目
西安市科技计划项目(21NYYF0013) （21NYYF0013）
国家自然科学基金原创探索计划项目(82350124) （82350124）
