动物医学进展2026，Vol.47Issue(5)：139-144,6.
青海某羊场绵羊流产衣原体的检测与鉴定
Detection and Identification of Chlamydia abortus in Aborted Sheep from a Farm in Qinghai Province
摘要
Abstract
To identify the causative agents of abortion in pregnant ewes on a sheep farm in Qinghai Province,sera,vaginal swabs,and aborted fetuses were collected from affected ewes.Brucella and Chlamydia infections were assessed using rose bengal plate agglutination test,indirect hemagglutination assay(IHA),PCR,and fluorescence quantitative PCR.The results revealed that 13 out of 15 serum samples tested positive for Chlamydia IHA,with two showing indeterminate results.PCR testing for Chlamydia revealed 14 positive swab samples and three positive aborted fetuses.Three strains of Chlamydia were suc-cessfully isolated from the stomach fluids of Chlamydia-positive aborted fetuses by inoculation with SPF chicken embryos,and the isolates were identified as Chlamydia abortus through sequencing of the ompA gene.All 15 serum samples were negative in the rose bengal plate agglutination test,and 15 swab samples and 3 aborted fetal samples were negative for Brucella using fluorescence quantitative PCR.These results indicate that Chlamydia abortus is the primary pathogen responsible for abortions in pregnant ewes on this farm,providing valuable reference for the epidemiology and prevention of Chlamydia abortus in sheep in Qinghai.关键词
流产衣原体/PCR/羊流产Key words
Chlamydia abortus/PCR/Sheep abortion分类
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杨怀星,王光华,张潇涵,马利青,李秀萍,简莹娜,胡勇,杨雪,龚婷婷..青海某羊场绵羊流产衣原体的检测与鉴定[J].动物医学进展,2026,47(5):139-144,6.基金项目
青海省"昆仑英才"乡村振兴人才项目(2022YFD1602304) （2022YFD1602304）
