大连工业大学学报2026，Vol.45Issue(2)：79-88,10.DOI:10.19670/j.cnki.dlgydxxb.2026.0201
药用植物中单萜吲哚生物碱的代谢工程调控策略研究进展
Research progress on metabolic engineering of monoterpenoid indole alkaloids in medicinal plants
摘要
Abstract
Monoterpenoid indole alkaloids(MIAs),a class of natural products with remarkable pharmacological activities,are widely distributed in multiple medicinal plant species of the Apocynaceae family.Significant application values of these compounds have been demonstrated in the treatment of anti-tumor,cardiovascular and cerebrovascular diseases,as well as central nervous system diseases.The biosynthetic pathways of MIAs in four representative medicinal plants,Catharanthus roseus,Camptotheca acuminata,Uncaria spp.and Melodinus yunnanensis.are systematically reviewed.The synthetic process of the common precursor strictosidine and the formation mechanism of product structural diversification are emphatically expounded,and the enzymatic functional differentiation and evolutionary path differences among different species in precursor generation and downstream modification are revealed.The core strategies of current metabolic engineering for regulating MIA biosynthesis are further summarized,including regulation of endogenous and exogenous enzyme expression,transcription factor regulation,gene silencing and editing,transporter-mediated regulation,and hormonal regulation,which provides theoretical support for efficient synthesis of high-value MIAs.In conclusion,the metabolic network of MIAs is complex and sophisticated.The combination of single-cell omics and synthetic biology technologies will open up new directions for the development and application of novel medicinal alkaloids.关键词
单萜吲哚生物碱/药用植物/生物合成途径/次生代谢调控Key words
monoterpenoid indole alkaloids/medicinal plants/biosynthetic pathway/secondary metabolism regulation分类
农业科技引用本文复制引用
李阳,陈馨圜,金朝霞..药用植物中单萜吲哚生物碱的代谢工程调控策略研究进展[J].大连工业大学学报,2026,45(2):79-88,10.基金项目
国家自然科学基金项目(42177112). （42177112）
