大连工业大学学报2026，Vol.45Issue(2)：89-96,8.DOI:10.19670/j.cnki.dlgydxxb.2026.0202
水稻秸秆腐熟菌株筛选及其复合菌系的构建
Screening of rice straw rotting strains and construction of composite strains
摘要
Abstract
To address the issues of prolonged decomposition time and poor decomposition effects associated with using single strains or composite microbial systems for straw treatment,strains with straw-degrading capabilities were screened based on the enzymatic activities of cellulase,laccase,xylanase,and lignin peroxidase as indicators.The selected strains were identified and antagonism tests were conducted,then their decomposition capabilities were determined.A total of 85 strains exhibiting enzymatic activities related to straw degradation were screened.Three straw-decomposing strains with no mutual antagonism and relatively high enzymatic activities were selected.Through molecular biological identification,all three were confirmed to belong to Bacillus genus.The results indicated that the cellulase and xylanase activities of strain L4 reached 0.235 U/mL and 1.335 U/mL,respectively.The laccase activity of strain L6 was measured at 0.711 U/mL,and the lignin peroxidase activity of strain X6 reached 4.301 U/mL.The decomposition experiment results demonstrated that the cellulase activity within the straw stack was increased by 53.47％,xylanase activity by 52.72％,laccase activity by 46.46％,and lignin peroxidase activity by 68.56％after 40 d compared with natural decomposition.The straw weight loss rate was increased by 16.06％,and the cellulose degradation rate was increased by 23.67％.When the decomposed straw was used as compost,the wheat seed germination rate was increased by 16％.The composite microbial formulation prepared from the screened strains and added to straw for decomposition was found to enhance the enzymatic activities within the straw stack,effectively promote straw decomposition,and improve crop growth.关键词
水稻秸秆/腐熟/菌株筛选/复合菌系Key words
rice straw/decomposition/strain screening/composite microbial system分类
生物科学引用本文复制引用
姜子豪,栾静,侯晓晴,宋忠振,俞志敏..水稻秸秆腐熟菌株筛选及其复合菌系的构建[J].大连工业大学学报,2026,45(2):89-96,8.基金项目
国家自然科学基金项目(31671796) （31671796）
辽宁省教育厅基本科研项目(LJ212410152061). （LJ212410152061）
