摘要
Abstract
To improve the yield of coenzyme Q10 produced by fermentation of Rhodobacter sphaeroides and meet the needs of industrial production,the optimal conditions for shake flask fermentation were determined through single-factor optimization and orthogonal experiments.The initial pH,glucose mass concentration of 50 g/L,potassium dihydrogen phosphate of 1.5 g/L,yeast extract powder of 1.0 g/L,and corn steep liquor powder of 4.0 g/L.Under this condition,102 g/L wet bacteria can be obtained by shake flask fermentation,and coenzyme Q10 production reaches 432 mg/L.Based on the optimization results of shake flasks,high initial concentration material fed-batch fermentation and low concentration material fed-batch fermentation were carried out in a 100 L automatically controlled fermentor.It is found that when the low concentration glucose and phosphorus fed-batch process were used,the glucose concentration was stably controlled below 15 g/L,and the phosphorus content was maintained below 0.25 g/L during fermentation.After 80 to 90 h of fermentation,the wet bacterial biomass of Rhodobacter sphaeroides could reach 248 g/L,the yield of coenzyme Q10 reached 3 221 mg/L,and the production intensity was 36.60 mg/(L·h).Compared with the initial high-concentration material process,the wet bacterial biomass increased by 64.2％,and the coenzyme Q10 yield increased by 374.4％.The fermentation kinetic models for bacterial growth,substrate consumption and coenzyme Q10 production were established,which can accurately predict the changes during fermentation.关键词
辅酶Q10/类球红细菌/低浓度物料流加/发酵工艺Key words
coenzyme Q10/Rhodobacter sphaeroides/low concentration fed-batch/fermentation process分类
轻工纺织
