分析测试学报2026，Vol.45Issue(5)：922-928,7.DOI:10.12452/j.fxcsxb.26020601
肌萎缩侧索硬化症患者血液外泌体的苏木化修饰研究
SUMOylation of Blood Exosomes in Amyotrophic Lateral Sclerosis
摘要
Abstract
The clinical diagnosis of ALS lacks early biomarkers,and the complexity of peripheral blood components makes it difficult to directly reflect pathological changes.Blood exosomes,which stably carry protein information from their source cells,provide a window for non-invasive disease monitoring.SUMOylation is a crucial post-translational modification that plays a key regulatory role in disease.However,the characteristics of SUMO-1 modification in the blood exosomes of ALS pa‑tients remain unclear.To address this,this study utilized an affinity ligand(CP-1)screened via phage display technology to synthesize a specific enrichment material(M2).Combined with LC-MS/MS,a proteomic analysis of SUMO-1 modifications was performed on blood exosomes from 4 early-stage and 4 mid-to-late-stage ALS patients.A total of 119 SUMO-1-modified proteins were identi‑fied.The overall abundance of these modified proteins was significantly higher in middle-to-late-stage patients compared to early-stage patients,and they were primarily enriched in the chemokine signal‑ing and HIF-1 signaling pathways.These findings suggest that exosomal SUMO-1 modification may be involved in the pathological process of ALS,providing new clues for understanding the disease mechanism.关键词
肌萎缩侧索硬化症/外泌体/苏木化修饰/噬菌体筛选/液相色谱-串联质谱Key words
amyotrophic lateral sclerosis/exosomes/SUMOylation/phage display/LC-MS/MS分类
化学化工引用本文复制引用
周凌竹,王存利,张晓雨,宋艳玲,卿光焱..肌萎缩侧索硬化症患者血液外泌体的苏木化修饰研究[J].分析测试学报,2026,45(5):922-928,7.基金项目
国家自然科学基金项目(22504140) （22504140）
