分析测试学报2026，Vol.45Issue(5)：930-939,10.DOI:10.12452/j.fxcsxb.25122304
代谢组学探讨布渣叶干预高脂血症小鼠降脂作用及精氨酸-脯氨酸-多胺代谢轴调控机制
Metabolomics Reveals Lipid-lowering Effects of Microctis Folium in Hyperlipidemic Mice via Modulation of the Arginine-Proline-Polyamine Axis
摘要
Abstract
This study employed a high-fat diet(HFD)-induced hyperlipidemia mice model to system‑atically evaluate the lipid-lowering and hepatoprotective effects of Microctis Folium and to elucidate its regulatory mechanism on the arginine-proline-polyamine metabolic axis using integrated untarget‑ed and targeted metabolomics.Serum untargeted metabolomics was performed using UPLC-QTOF-MS/MS to screen for differential metabolites and conduct pathway enrichment analysis.Subsequently,targeted metabolomics based on UPLC-TQ-MS/MS was employed to quantify key metabolites includ‑ing L-arginine,proline,creatine,and polyamines(spermidine and spermine).Microctis Folium significantly reduced TC,TG,and LDL-C levels and decreased ALT and AST while increasing HDL-C across dose groups.Untargeted metabolomics revealed broad metabolic disturbances induced by HFD,with significant enrichment of arginine and proline metabolism;differential metabolites mainly involved amino acids and derivatives,polyamines,creatine-related metabolites,as well as lipids and organic acids.Microctis Folium treatment globally reversed these metabolic abnormalities.Targeted quantification further confirmed that L-arginine,proline,creatine,and polyamines(spermine/spermidine)were decreased in the model group,and Microctis Folium markedly restored their levels toward those of controls.Collectively,Microctis Folium exhibits pronounced lipid-lower‑ing and hepatoprotective activities,which may be mediated by modulation of the arginine-proline-polyamine metabolic axis and associated metabolic remodeling of nitrogen and energy metabolism,thereby promoting restoration of metabolic homeostasis.These findings provide metabolomics-based evidence for the mechanism of action of Microctis Folium and support future screening of its bioactive constituents and translational development.关键词
布渣叶/高脂血症/精氨酸-脯氨酸-多胺代谢/超高效液相色谱-四极杆-飞行时间质谱/超高效液相色谱-三重四极杆串联质谱Key words
Microcos Folium/hyperlipidemia/arginine-proline-polyamine metabolism/UPLC-QTOF-MS/MS/UPLC-TQ-MS/MS分类
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肖观林,陈婉纯,刘焰嫦,欧阳云徽,张靖年,陈伟韬,毕晓黎..代谢组学探讨布渣叶干预高脂血症小鼠降脂作用及精氨酸-脯氨酸-多胺代谢轴调控机制[J].分析测试学报,2026,45(5):930-939,10.基金项目
广东省基础与应用基础研究基金项目(2024A1515012809) （2024A1515012809）
广州地区中医药重大科技项目(2025QN003) （2025QN003）
广东省中医药局科研项目(20251034) （20251034）
广州中医药大学校院联合科技创新基金(GZYSE2024G07) （GZYSE2024G07）
2025年度国家中医药管理局-广东省国家中医药综合改革示范区科技共建项目(GZY-KJS-GD-2025-017) （GZY-KJS-GD-2025-017）
