灌溉排水学报2026，Vol.45Issue(5)：50-56,7.DOI:10.13522/j.cnki.ggps.2025231
滴灌条件下生物炭对土壤硝化微生物的影响
Effects of biochar on soil nitrifying microorganisms under drip irrigation
摘要
Abstract
[Objective]Biochar application has been reported to be capable of modifying soil physicochemical properties and microbial communities,yet its effects on nitrifying microorganisms under different irrigation conditions remain elusive.This paper investigates the combined influence of biochar addition and different irrigation methods on soil nitrifying microorganisms.[Method]The experiment was conducted in a field in northern China.It consisted of three biochar application treatments:0(CK),10(LB)and 20 t/hm2(HB),and two irrigation methods:drip irrigation(D)and conventional furrow irrigation(F).Nitrifying microorganisms in each treatment were measured using quantitative PCR(qPCR).We also measured NH4+-N and NO3--N contents,as well as gene abundances of ammonia-oxidizing archaea(AOA)and ammonia-oxidizing bacteria(AOB)in the soils.[Result]Compared with furrow irrigation,drip irrigation significantly increased AOA gene abundance and soil NO3--N content,while reducing soil NH4+-N content,indicating that optimized water and oxygen conditions under drip irrigation enhanced the activity and proliferation of nitrifying microorganisms.Under drip irrigation,biochar addition reduced AOB gene abundance and soil NH4+-N content,likely due to substrate adsorption and modification of microbial habitat.Applying 20 t/hm2 of biochar combined with drip irrigation increased NO3--N content and promoted overall nitrification,while increasing AOA abundance and reducing AOB abundance.This suggests that AOA plays the dominant role in nitrification.[Conclusion]Applying 20 t/hm2 of biochar combined with drip irrigation promotes soil nitrification primarily through enhancing the AOA population.In practice,irrigation and fertilization strategies can be adjusted to maintain nitrogen balance and optimize crop productivity.关键词
滴灌/生物炭/硝化过程/微生物Key words
drip irrigation/biochar/nitrification process/microorganism分类
农业科技引用本文复制引用
冯苗苗,刘杰云,郭金秀,卞雅琪,孙芮晗,邱虎森..滴灌条件下生物炭对土壤硝化微生物的影响[J].灌溉排水学报,2026,45(5):50-56,7.基金项目
安徽省教育厅高校科学研究项目(2022AH030137) （2022AH030137）
安徽省质量工程项目(2024aijy384) （2024aijy384）
农业农村部节水灌溉工程重点实验室开放课题(IFI2023KF01) （IFI2023KF01）
宿州学院大学生创新创业项目(YBXM23-193) （YBXM23-193）
