广西师范大学学报（自然科学版）2026，Vol.44Issue(3)：192-203,12.DOI:10.16088/j.issn.1001-6600.2025080601
氯化钙浸渍对冻干霍山石斛品质影响及其作用机制研究
Calcium Chloride Pretreatment in Freeze-Dried Dendrobium Huoshanense:Quality Effects and Action Mechanism
摘要
Abstract
To improve the freeze-drying quality of fresh Dendrobium huoshanense,this study explored the effects of calcium chloride(CaCl2)immersion pretreatment on the physical properties,active components,and underlying mechanisms of freeze-dried samples.The results showed that appropriate CaCl2 treatment enhanced the moisture characteristics,texture,color,and polysaccharide content of freeze-dried samples.Among all treatments,the 3.0%CaCl2 group exhibited the best performance,with a moisture content of 7.40%±0.19%,rehydration ratio of 3.02±0.03,hardness of 2.70±0.23 N,crispness of 1.34±0.11 N·S,and a minimal color difference(ΔE)of 2.02±0.07.Its polysaccharide content reached 32.47%±0.73%,an increase of 10.3%was made compared with the untreated control.Subsequently,the differences between extracted dendrobium pectin and calcium pectate prepared by reacting with CaCl2 were analyzed.FT-IR analysis revealed a shift of the characteristic carboxylate peak from 1 591.2 cm-1 to approximately 1 621.1 cm-1,and NMR spectra showed significant changes in proton signals.The weight-average molecular weight of pectin(414 777 g/mol)increased gradually with CaCl2 concentration(1.5%-6.0%)to 529 211 g/mol.SEM observations showed that the microstructure of calcium pectate changed from loose and collapsed to dense and compact.These findings indicate that Ca2+forms a stable"egg-box"crosslinking structure with pectin in the cell wall,which is the core mechanism for quality improvement.This study provides theoretical and technical support for the high-quality and value-added processing of Dendrobium huoshanense.关键词
霍山石斛/真空冷冻干燥/氯化钙浸渍/果胶酸钙/质构特性Key words
Dendrobium huoshanense/vacuum freeze-drying/CaCl2 immersion pretreatment/calcium pectate/textural properties分类
医药卫生引用本文复制引用
胥凯,冉庆念,黄斌,张文成,何祥林,吴泽宇,惠爱玲..氯化钙浸渍对冻干霍山石斛品质影响及其作用机制研究[J].广西师范大学学报（自然科学版）,2026,44(3):192-203,12.基金项目
安徽省质量基础设施标准化专项(2022MKS08) （2022MKS08）
