动物医学进展2026，Vol.47Issue(5)：1-10,10.
基于转录组分析2株单增李斯特菌的环境胁迫抗性机制
Transcriptome-based Analysis of Environmental Stress Resistance Mechanism in Two Listeria monocytogenes Strains
摘要
Abstract
To investigate the stress resistance mechanisms of Listeria monocytogenes(LM)strains LM66-3 and LM83-1,the standard strain ATCC 19111 was used as a control.The growth performance of the two strains was evaluated under various stress conditions,including low temperature(4℃),strong acid(pH 3.0),strong base(pH 9.0),high salt(10％NaCl),and oxidative stress(10 mmol/L H2O2),and their metabolic pathways were analyzed using transcriptomic sequencing.The results demonstrated that the sur-vival capacity of the isolates was significantly higher than that of the standard strain under various stress conditions.Transcriptome sequencing revealed that 475 and 513 differentially expressed genes(DEGs)were identified in LM66-3 and LM83-1,respectively.GO analysis revealed that DEGs were significantly enriched in functional categories related to bacterial flagella,glutaminase complex,peptidoglycan transmembrane transporter activity,and glycine decarboxylation.KEGG enrichment analysis indicated that DEGs were pri-marily associated with metabolic pathways such as methyl butanoate metabolism,ABC transporter system,glycolysis/gluconeogenesis pathway,organic selenium compound metabolism,quorum sensing system(QS),and two-component system(TCS).RT-qPCR validation of differentially expressed genes confirmed consistency with the transcriptomic findings.This study demonstrates that LM66-3 and LM83-1 enhance their ability to resist environmental stress through the regulation of specific pathways,providing a theoreti-cal foundation for further exploration of Listeria monocytogenes stress resistance mechanisms.关键词
单增李斯特菌/生长曲线/差异表达基因/转录组测序Key words
Listeria monocytogenes/Growth curve/Differentially expressed genes/Transcriptome sequencing分类
农业科技引用本文复制引用
张坤中,薛慧文,苟惠天,田常青,曹青,崇倩,盖文燕,何佳兵,程明霞,王佳雨,王中龙..基于转录组分析2株单增李斯特菌的环境胁迫抗性机制[J].动物医学进展,2026,47(5):1-10,10.基金项目
国家自然科学基金项目(31960726,32060822) （31960726,32060822）
甘肃省现代丝路寒旱农业科技支撑项目(GSLK-2022-17) （GSLK-2022-17）
甘肃省农业科技支撑项目(LJZC-2024-20) （LJZC-2024-20）
甘肃省青年博士支持项目(2024QB-077) （2024QB-077）
山东畜牧兽医职业学院自然科学类重点课题(2022zrkxzd005) （2022zrkxzd005）
