动物医学进展2026，Vol.47Issue(5)：11-16,6.
siRNA靶向干扰EGr_09888对细粒棘球绦虫原头蚴体外发育的影响
Effect of siRNA-targeted Interference on the in vitro Development of Echinococcus granulosus Protoscolices EGr_09888
摘要
Abstract
The objective of this study is to investigate the influence of Echinococcus granulosus EGr_09888 on the development of protoscoleces(PSCs).The optimal EGr_09888 siRNA fragment was screened by RT-qPCR,and siRNA was electroporation into PSCs cultured in vitro for 3 days.PSCs were collected at 1 d,3 d and 7 d,and the development status of PSCs was observed,The expression levels of EGr_09888 gene and protein in PSCs were analyzed by RT-qPCR and Western blot.The results showed that the optimal siRNA fragment was EGr_09888-siRNA-3.RT-qPCR analysis showed that the EGr_09888 gene of PSCs was interfered in different degrees at 1 d,3 d and 7 d after siRNA electrotransfection(1 d,3 d P＜0.000 1,7 d P＜0.001).The expression of EGr_09888 protein in PSCs at 1 d,3 d and 7d was signifi-cantly decreased by SDS-PAGE and Western blot(1 d,3 d P＜0.000 1,7 d P＜0.05).After the expression of EGr_09888 gene and protein was down-regulated,PSCs tended to develop toward the cyst.This study utilizes RNAi technology to target and silence the EGr_09888 gene in PSCs,revealing for the first time its regulatory role in PSCs development.This provides a theoretical basis for elucidating the developmental mechanism of Echinococcus granulosus and formulating prevention and control strategies.关键词
细粒棘球绦虫/原头蚴/EGr_09888/siRNA干扰/包囊化发育Key words
Echinococcus granulosus/Protoscolex/EGr_09888/siRNA interference/Cyst development分类
农业科技引用本文复制引用
潘星羽,吾力江·卡马力,班万里,刘帅,王冰洁,喀迪尔丁·艾尔肯,慕永辉,张壮志,赵莉..siRNA靶向干扰EGr_09888对细粒棘球绦虫原头蚴体外发育的影响[J].动物医学进展,2026,47(5):11-16,6.基金项目
新疆维吾尔自治区自然科学基金项目(2022D01B177) （2022D01B177）
国家重点研发计划项目(2023YFD1802401) （2023YFD1802401）
新疆维吾尔自治区公益性科研院所项目(KY202462) （KY202462）
