动物医学进展2026，Vol.47Issue(5)：17-23,7.
兔抗猪PD-L1多克隆抗体的制备及初步应用
Preparation and Preliminary Application of Porcine PD-L1 Polyclonal Antibody
摘要
Abstract
Programmed cell death ligand-1(PD-L1)modulates immune responses by regulating T cell functions.Due to structural divergence among porcine,human,and murine PD-L1,and the lack of specific antibodies,this study cloned the porcine PD-L1 gene fragment from PK-15 cells.Sequence analysis revealed it was identical to the porcine PD-L1 gene sequence in the NCBI database.Its secondary structure was pri-marily composed of beta-sheets and random coils.We constructed a pET-28a recombinant vector to express the protein.New Zealand white rabbits were immunized with the purified protein,successfully generating polyclonal antibodies that specifically detect porcine PD-L1 in Western blot assays.Utilizing this antibody,the study demonstrated that overexpression of PD-L1 in porcine PK-15 cells facilitates the replication of classical swine fever virus(CSFV),whereas knockdown of PD-L1 inhibits viral replication.This indicates that PD-L1 plays a significant role in CSFV infection.These findings provide foundational data for subsequentresearch into the function of PD-L1 during viral infections.关键词
猪/细胞程序性死亡配体1/新西兰兔/多克隆抗体/猪瘟病毒Key words
Pig/PD-L1/Rabbit/Polyclonal antibody/CSFV分类
农业科技引用本文复制引用
全钺涵,李宁,罗弼豪,郭抗抗..兔抗猪PD-L1多克隆抗体的制备及初步应用[J].动物医学进展,2026,47(5):17-23,7.基金项目
国家自然科学基金项目(32272997) （32272997）
