动物医学进展2026，Vol.47Issue(5)：24-28,5.
鸡传染性喉气管炎病毒荧光RAA检测方法的建立与应用
Establishment and Application of a Fluorescent Recombinase Aided Amplification Assay for Detection of Infectious Laryngotracheitis Virus
摘要
Abstract
In order to meet the demand for rapid detection of infectious laryngotracheitis virus(ILTV),a real-time fluorescence isothermal detection assay was established based on the recombinant aided amplifica-tion(RAA)in this study.The detection efficiency of the fluorescence RAA assay was evaluated.The results showed that the fluorescence RAA assay completed the detection in 20 minutes at a constant temperature 39℃ and the detection results could display in real time.The assay had no cross-reaction with other poultry respiratory pathogens,and the detection limit of this method reached 1 copy/μL.A total of 396 swab samples from poultry were detected both by fluorescence RAA assay and standard assay.Compared with the standard assay,the coincident rate for positive of fluorescence RAA assay was 100％,and for negative was 99.74％.In conclusion,the fluorescence RAA assay is characteristic of time saving,high specificity and sensitivity,which is suitable for the rapid detection of ILTV in clinical samples.The fluorescence RAA assay for ILTV in this study provided a new method for the rapid screening and surveillance.关键词
鸡传染性喉气管炎病毒/重组酶介导等温扩增技术/检测Key words
Infectious laryngotracheitis virus/Recombinant aided amplification/Detection分类
农业科技引用本文复制引用
王素春,金雅文,祁倩,周凯钰太,潘俊慧,隋金钰,魏世萌,李超,王楷宬..鸡传染性喉气管炎病毒荧光RAA检测方法的建立与应用[J].动物医学进展,2026,47(5):24-28,5.基金项目
国家重点研发计划项目(2023YFD1800604) （2023YFD1800604）
