动物医学进展2026，Vol.47Issue(5)：29-34,6.
新疆部分地区犊牛肺炎链球菌分离鉴定及其部分生物学特性分析
Isolation and Identification of Streptococcus pneumoniae from Calves in Some Areas of Xinjiang and Analysis of Some Biological Characteristics
摘要
Abstract
In order to investigate the epidemic situation and biological characteristics of Streptococcus pneu-moniae in calves in some areas of Xinjiang,the nasal swabs of calves with pneumonia in some areas were collected aseptically in this study.Streptococcus was isolated and identified by routine method,and its drug resistance was detected by K-B method.The virulence genes and drug resistance of the isolated strains were detected by PCR.One isolate carrying multiple virulence was selected for the median lethal dose test in mice.The results showed that a total of 10 strains of streptococci were isolated and identified from the nasal swabs of calves with pneumonia in three areas of Xinjiang,with a separation rate of 8.33％(5/60)in Aksu area,3.75％(5/133)in Yili area,and no detection in 25 samples from Changji area.Ten strains of Streptococcus showed multi-drug resistance,mainly carrying tetA,floR and other drug resistance genes.Five virulence genes(nanA,LytA,pspA,psaA,and clpP)were detected.The LD50 of the isolated strain XJ-AKS-S-15 was 2.18×106 CFU/mL.Streptococci could be isolated from the heart blood of the dead mice,and obvious pathological changes were observed in the lung and spleen tissue sections of the mice.These findings suggest that streptococci pose a certain potential threat to calves.关键词
犊牛/链球菌/毒力基因/耐药性Key words
calf/Streptococci/Virulence gene/Drug resistance分类
农业科技引用本文复制引用
刘怡帆,孙雪,王一霖,张凌,秦天,王伟,付强,刘虹,苏战强..新疆部分地区犊牛肺炎链球菌分离鉴定及其部分生物学特性分析[J].动物医学进展,2026,47(5):29-34,6.基金项目
新疆维吾尔自治区重大科技专项-牛常见多发病防控关键技术研究与集成示范资助项目(2023A02007-2) （2023A02007-2）
新疆维吾尔自治区三农骨干人才资助项目(2023SNGGNT005) （2023SNGGNT005）
