动物医学进展2026，Vol.47Issue(5)：43-54,12.
基于代谢组学研究住肉孢子虫对藏羊肌肉氨基酸的影响
Effects of Sarcocystis on Amino Acids in Tibetan Sheep Muscle based on Metabonomics
摘要
Abstract
To provide reference for identifying potential biomarkers of Sarcocystis,the changes of amino acids in the muscles of Tibetan sheep infected with Sarcocystis were explored based on qualcomm quantitative target metabonomics.A total of 25 different amino acids were screened from the muscles of Tibetan sheep in high infection group,middle infection group and non-infection group.Among them,22 amino acids in non-infection group and high infection group were down-regulated,and 3 amino acids were obviously up-regulated.Compared with middle infection group,19 amino acids were down-regulated and 6 amino acids were obviously up-regulated.Based on the analysis of differences in amino acid content among different groups,significant differences in L-proline andL-glutamic acid were found among the non-infected group,the moderately infected group,and the highly infected group(P＜0.05).By grouping comparison,it was found that there was a significant difference between the two groups in L-glutamic acid(P＜0.01)and glycine(0.01＜P＜0.05).There is a positive correlation between amino acids such as L-glutamic acid and glycine,L-glutamine,L-serine and L-proline between the non-infected group and the highly infected group,while there is a significant negative correlation between L-alanine and L-tryptophan,and 5-hydroxylysine and 3-methyl-L-histidine between the non-infected group and the moderately infected group,showing some differences compared with different infection intensity groups.The results provide a molecular basis for the diagnosis and detection of Sarcocystis based on L-glutamate,glycine,and L-proline.关键词
藏羊/住肉孢子虫/腿部肌肉/差异代谢物/高通量靶标代谢组学Key words
Tibetan sheep/Sarcocystis/Leg muscle/Differential metablites/Qualcomm mass target metabonomics分类
农业科技引用本文复制引用
裴晓双,龙敏,单曲拉姆,米玛卓嘎,董海龙,夏晨阳..基于代谢组学研究住肉孢子虫对藏羊肌肉氨基酸的影响[J].动物医学进展,2026,47(5):43-54,12.基金项目
西藏自治区"十四五"重大科技专项(XZ202101ZD0001N) （XZ202101ZD0001N）
