动物医学进展2026，Vol.47Issue(5)：54-62,9.
血清4型禽腺病毒Fiber蛋白抗原表位串联表达及其在抗体检测中的应用
Tandem Expression of Antigenic Epitopes of Fiber Proteins of Fowl Adenovirus Type 4 and Its Application in Antibody Detection
摘要
Abstract
This study aims to explore the tandem expression of multiple antigenic epitope peptides of Fiber-1 and Fiber-2 proteins of the fowl adenovirus type 4(FAdV-4)using an Escherichia coli expression sys-tem,and to establish a highly sensitive and specific indirect enzyme-linked immunosorbent assay(ELISA)for detecting antibodies against FAdV-4 using the expressed recombinant protein as a coating antigen.Bioinformatics and immunoinformatics software were used to predict and screen 5 antigenic epitopes each from FAdV-4 Fiber-1 and Fiber-2 proteins,the tandem polypeptide sequences of the 10 antigenic epitopes(named F1/2)were artificially synthesized and expressed in E.coli expression system to obtain the F1/2 recombinant protein.Using the purified F1/2 recombinant protein as a coating antigen,an indirect ELISA for detecting FAdV-4 antibodies was established after optimizing the reaction conditions.Results of speci-ficity tests showed that the established ELISA could specifically detect FAdV-4 antibodies without cross-reactivity with positive sera of 8 other pathogens,such as NDV,ARV,etc.The detection limit of the ELISA for FAdV-4 positive serum was 1∶12,800 dilution,and its coefficients of variation in intra-assay and inter-assay reproducibility tests were both below 5％.In conclusion,this study has successfully established an in-direct ELISA method for detecting FAdV-4 antibodies,which provides a valuable tool and support for the epidemiological investigation and antibody monitoring of FAdV-4.关键词
血清4型禽腺病毒/纤突蛋白/抗原表位/原核表达/酶联免疫吸附试验Key words
Fowl adenovirus type 4/Fiber/Antigenic epitope/Prokaryotic expression/ELISA分类
农业科技引用本文复制引用
陈悦,张莉,鄢明华,郭笑然,刘佳俐,田向学,张娜,李鹏飞,崔松奇,师延峰,王利丽..血清4型禽腺病毒Fiber蛋白抗原表位串联表达及其在抗体检测中的应用[J].动物医学进展,2026,47(5):54-62,9.基金项目
天津市科技计划项目(23YDTPJC00050) （23YDTPJC00050）
天津市农业科学院博士创新资助项目(TJNKY012542) （TJNKY012542）
天津市农业科学院种业创新专项(2025ZYCX004) （2025ZYCX004）
