甘肃中医药大学学报2026，Vol.43Issue(2)：7-13,7.DOI:10.16841/j.issn1003-8450.2026.02.02
护卵汤加减方对多囊卵巢综合征模型大鼠卵巢组织PI3K、AKT蛋白表达及血糖、血脂、激素水平的影响
Effect of Modified Huluan Tang(护卵汤加减方)on PI3K and AKT protein expression in ovarian tissue and blood glucose,blood lipids,and hormone levels in PCOS model rats
摘要
Abstract
Objective To investigate the mechanism of Modified Huluan Tang(护卵汤加减方)in intervening with polycystic ovary syndrome(PCOS)based on the phosphoinositide 3-kinase(PI3K)/protein kinase B(AKT)signaling pathway.Methods Sixty-two SD rats were randomly divided into four groups using a random number ta-ble:a blank group(n=8),a model group(n=18),a western medicine group(n=18),and a traditional Chinese medicine(TCM)group(n=18).The PCOS rat model was established by continuous gavage of letrozole combined with 1％carboxymethyl cellulose.After successful modeling,the blank and model groups received 0.9％normal sa-line via gavage,the western medicine group received Diane-35 solution,and the TCM group received concentrated Modified Huluan Tang,all for 21 consecutive days.Fasting blood glucose(FBG),triglyceride(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),serum hormone[follicle-stimulating hormone(FSH),estradiol(E2),luteinizing hormone(LH),anti-Müllerian hormone(AMH),testosterone(T),insulin(INS)]levels,and PI3K and AKT protein expression levels in ovarian tissue were com-pared.Pathological changes in ovarian,uterine,and liver tissues were observed via hematoxylin-eosin(HE)stai-ning.Results Compared with the blank group,the model group showed significantly increased FBG,TG,TC,LDL-C,and serum LH,AMH,T,and INS levels,while HDL-C,serum FSH,E2 levels,and PI3K and AKT protein relative expression level in ovarian tissue were significantly decreased(P＜0.05 or P＜0.01).Compared with the model group,the western medicine and TCM groups exhibited significantly reduced FBG,TG,TC,LDL-C,and serum LH,AMH,T,and INS levels,while HDL-C,serum FSH,E2 levels,and PI3K and AKT protein relative expression level were significantly increased(P＜0.05 or P＜0.01).The western medicine and TCM groups ameliorated pathological changes in ovarian,uterine,and liver tissues to varying degrees,effectively improving ovarian morphology,enriching the granulosa cell layer with orderly distribution,and promoting oocyte maturation.Conclusion Modified Huluan Tang may inhibit apoptosis of ovarian granulosa cells in PCOS model rats by activating the PI3K/AKT signaling pathway,thereby improving ovulatory function.关键词
多囊卵巢综合征/护卵汤加减方/磷脂酰肌醇3-激酶/蛋白激酶B/空腹血糖/血脂/激素/大鼠Key words
polycystic ovary syndrome/modified Huluan Tang(护卵汤加减方)/phosphoinositide 3-kinase/protein kinase B/fasting blood glucose/blood lipids/hormones/rats分类
医药卫生引用本文复制引用
杨永琴,魏本君,申剑,王帅哲,马睿玲,胡杨,杨燕..护卵汤加减方对多囊卵巢综合征模型大鼠卵巢组织PI3K、AKT蛋白表达及血糖、血脂、激素水平的影响[J].甘肃中医药大学学报,2026,43(2):7-13,7.基金项目
甘肃省中医药管理局科研项目(GZK-2018-10) （GZK-2018-10）
敦煌医学与转化教育部重点实验室开放基金项目(DHYX17-03) （DHYX17-03）
甘肃省高等学校创新基金科研项目(2022B-120) （2022B-120）
甘肃中医药大学引进人才项目(2018YJRC-05). （2018YJRC-05）
