基于AMPK-SIRT3-BNIP3通路探讨益气养阴活血方对糖尿病肾病大鼠作用机制
Effects of Yiqi Yangyin Huoxue Recipe on diabetic kidney disease via regulating AMPK-SIRT3-BNIP3 pathway in a rat model
摘要
Abstract
Objective:To explore the protective mechanism of Yiqi Yangyin Huoxue Recipe on diabetic kidney disease(DKD)rats based on AMPK-SIRT3-BNIP3 signaling pathway.Methods:A total of 42 SPF-grade male SD rats(with body weight ranging from 200~220 g)were used.After 1 week of environmental adaptation,the rats were divided into a blank group(n=6)and a modeling group(n=36).The two groups were fed with normal diet and high-fat/high-sugar diet respectively.A sin-gle intraperitoneal injection of streptozotocin(35 mg/kg)was used to establish the DKD model in the modeling group.The rats that were successfully induced were randomly divided into a valsartan(Diovan)group,a Yiqi Yangyin Huoxue Recipe equal-dose group,a Yiqi Yangyin Huoxue Recipe high-dose group,and a model group.The rats in each treatment group were given the corre-sponding drug doses by gavage,while the blank group and the model group were given the same volume of distilled water.The treatment lasted for 7 weeks.The 24-hour urinary protein(24 h-UTP)and serum biochemical indexes of the rats were detected by biochemical tests,including renal function indexes(SCr and BUN),liver function indexes(ALB and ALT),and glycolipid me-tabolism indexes(GLU,TC,and TG);H&E staining was used to observe the renal pathology;Western blot was used to detect the protein expression levels of AMPK,SIRT3,and BNIP3 in renal tissue;RT-PCR was used to detect the mRNA expression levels of AMPK,SIRT3,and BNIP3 in renal tissue.Results:Compared to the blank group,the levels of ALT,Scr,BUN,TC,TG,GLU,24 h-UTP,and renal tissue BNIP3 protein and mRNA expression levels in the model group rats were signifi-cantly increased(P<0.05),while the levels of ALB and renal tissue SIRT3 and AMPK proteins and mRNA expression levels were significantly decreased(P<0.05),and the renal pathological damage was severe.Compared to the model group,the levels of ALT,Scr,BUN,TC,TG,GLU,24 h-UTP,and renal tissue BNIP3 protein and mRNA expression levels in the treatment groups significantly decreased(P<0.05),while ALB level and renal tissue AMPK and SIRT3 protein and mRNA expression lev-els significantly increased(P<0.05),and the renal pathological damage was significantly alleviated.There was no statistically sig-nificant difference among the treatment groups(P>0.05).Conclusion:The Yiqi Yangyin Huoxue Recipe can effectively reduce 24 h-UTP in DKD rats,protect renal function,and improve renal pathology,thereby delaying the occurrence and development of DKD.The mechanism may be related to the regulation of the AMPK-SIRT3-BNIP3 pathway in renal tissue.关键词
益气养阴活血方/糖尿病肾病/AMPK-SIRT3-BNIP3/机制研究Key words
Yiqi Yangyin Huoxue Recipe/Diabetic kidney disease/AMPK-SIRT3-BNIP3/Mechanism分类
医药卫生引用本文复制引用
李慧聪,邢静静,宋珂,陈晨晨,姜浩然,宋纯东..基于AMPK-SIRT3-BNIP3通路探讨益气养阴活血方对糖尿病肾病大鼠作用机制[J].海南医科大学学报,2026,32(8):595-601,7.基金项目
This study was supported by the National Natural Science Foundation of China(82074493) （82074493）
Research Special Project of the National Clinical Research Base for Traditional Chinese Medicine of Henan Provincial Health Commission(2022JDZX003) （2022JDZX003）
Major Special Projects for Traditional Chinese Medicine Scientific Research in Henan Province(2023ZYZD02) （2023ZYZD02）
Henan Province Traditional Chinese Medicine Discipline Leading Talent Project[Yuwei Traditional Chinese Medicine Letter(2021)No.8] （2021）
"Double First-Class"Creation Project in Henan Province(HSRP-DFCTCM-2023-3-07) 国家自然科学基金(82074493) （HSRP-DFCTCM-2023-3-07）
河南省卫健委国家中医临床研究基地科研专项(2022JDZX003) （2022JDZX003）
河南省中医药科学研究重大专项(2023ZYZD02) （2023ZYZD02）
河南省中医药学科领军人才项目[豫卫中医函(2021)8号] （2021）
河南省"双一流"创建工程(HSRP-DFCTCM-2023-3-07) （HSRP-DFCTCM-2023-3-07）
