华北农学报2026，Vol.41Issue(2)：1-11,11.DOI:10.7668/hbnxb.20196210
RNA-Seq技术鉴定大豆雄性不育候选基因
Identification of Candidate Genes for Male Sterile in Soybean by RNA-Seq
摘要
Abstract
The large-scale production of soybean hybrids and the utilization of hybrid vigor are key to germ-plasm resource innovation and variety improvement.By mining and utilizing soybean male sterility genes,more ad-vantageous soybean varieties can be cultivated,thereby improving soybean yield and quality.This study selected the male sterile mutant population that appeared in the hybrid offspring of Baoquanling green soybean(female parent)and Heinong 44(male parent)as the experimental material.Flower buds were selected from soybean fertile and ster-ile plants,the anthers and pollen cytology were observed,and a high-throughput sequencing platform was used to perform transcriptome sequencing on the flower and anther tissues of soybean fertile and sterile plants.Annotated,screened and analyzed the sequencing results.The results indicated that abnormal development of the tapetum layer and high pollen sterility in sterile plants were important causes of male infertility.The pentose and glucuronate inter-conversion pathway(ko00040:Pentose and glucuronate interconversion)was a key pathway affecting soybean fertili-ty,and the genes encoding pectin methylesterase(PEM)and pectin lyase(PL)were downregulated in expression.The differentially expressed gene protein interaction network indicated that the pectin lyase gene was a key gene affecting the interconversion pathway between pentose and glucuronic acid.The determination of pectin lyase con-tent showed that the pectin lyase content in sterile plant anthers was significantly reduced.The qRT-PCR detection results were consistent with the RNA-Seq sequencing results.It is speculated that the downregulation of these genes may cause a decrease in pectin lyase activity,thereby affecting the development of the tapetum and leading to male infertility.关键词
大豆/雄性不育/转录组学/绒毡层/果胶裂解酶Key words
Soybean/Male infertility/Transcriptomics/Tapetum/Pectate lyase分类
农业科技引用本文复制引用
陈天宇,徐超,赵亮,王子烨,张琦,杜吉到..RNA-Seq技术鉴定大豆雄性不育候选基因[J].华北农学报,2026,41(2):1-11,11.基金项目
黑龙江省"双一流"新一轮建设学科协同创新成果建设项目"粮食作物绿色低碳"(LJGXCG2022-107) （LJGXCG2022-107）
黑龙江省"双一流"新一轮建设学科协同创新成果建设项目(LJGXCG2022-111) （LJGXCG2022-111）
