广州中医药大学学报2026，Vol.43Issue(5)：1332-1340,9.DOI:10.13359/j.cnki.gzxbtcm.2026.05.027
大蒜素调控CYP1B1-AS1/HSPA1B/ROS通路对糖尿病肾病大鼠焦亡的影响
Effects of Allicin Regulating the CYP1B1-AS1/HSPA1B/ROS Pathway to Inhibit Pyroptosis in Diabetic Nephropathy Rats
摘要
Abstract
Objective To investigate the therapeutic effects and underlying mechanism of allicin on type 2 diabetic nephropathy(DN).Methods Twenty-seven male SD rats were randomly divided into a sham-operated group,a model group,and an allicin group,with 9 rats in each group.The DN model was established in rats from the model and allicin groups.After successful modeling,the groups received respective interventions.Renal function indicators and oxidative stress markers were assessed.Reactive oxygen species(ROS)level in rat kidney tissues was detected using a fluorescent probe method.Renal histopathology was observed by periodic acid-Schiff(PAS)staining and hematoxylin-eosin(HE)staining,and the ultrastructure of renal tissue cells was examined by transmission electron microscopy.Western Blot analysis was employed to detect pyroptosis-related proteins[gasdermin E(GSDME),NLR family pyrin domain containing 1(NLRP1),NLR family pyrin domain containing 3(NLRP3),caspase-1,and heat shock protein family A member 1B(HSPA1B)]in renal tissues.Quantitative real-time reverse transcription polymerase chain reaction(qRT-PCR)was used to measure the mRNA expression level of cytochrome P450 family 1 subfamily B member 1 antisense RNA 1(CYP1B1-AS1).RNA immunoprecipitation(RIP)assay was performed to validate the interaction between HSPA1B and CYP1B1-AS1.Results Compared with the sham-operated group,the model group exhibited significantly abnormal renal function indicators and oxidative stress levels,marked inflammatory infiltration,glycogen deposition,and characteristic features of pyroptosis in renal tissues.ROS level in renal tissues was significantly elevated,accompanied by increased protein expression levels of pyroptosis-related markers(GSDME,NLRP1,NLRP3,Caspase1,HSPA1B)and upregulation of CYP1B1-AS1 mRNA expression(P＜0.05).In contrast,compared with the model group,the allicin-treated group demonstrated significantly improved renal function and oxidative stress parameters,alleviated renal inflammatory infiltration,glycogen deposition,and pyroptotic features.ROS level in renal tissues was notably reduced,and the expression levels of pyroptosis-related proteins(GSDME,NLRP1,NLRP3,HSPA1B,Caspase1)and CYP1B1-AS1 mRNA were significantly downregulated(P＜0.05).RIP assay confirmed the interaction between HSPA1B and CYP1B1-AS1.Conclusion Allicin can mitigate the progression of DN by regulating the CYP1B1-AS1/HSPA1B/ROS pathway,and alleviating oxidative stress damage and pyroptosis in renal tissues.关键词
大蒜素/糖尿病肾病/CYP1B1-AS1/HSPA1B/焦亡/氧化应激/大鼠Key words
allicin/diabetic nephropathy(DN)/CYP1B1-AS1/HSPA1B/pyroptosis/oxidative stress/rats分类
医药卫生引用本文复制引用
沈金峰,谢继芬,胡芳,王福珍,邓金秀,吴森超,江辉..大蒜素调控CYP1B1-AS1/HSPA1B/ROS通路对糖尿病肾病大鼠焦亡的影响[J].广州中医药大学学报,2026,43(5):1332-1340,9.基金项目
福建省自然科学基金项目(编号:2023J011898、2024J011612) （编号:2023J011898、2024J011612）
龙岩市科技计划项目(编号:2024LYF17034) （编号:2024LYF17034）
福建医科大学启航基因项目(编号:2022QH1340) （编号:2022QH1340）
