华北农学报2026，Vol.41Issue(2)：12-20,9.DOI:10.7668/hbnxb.20196133
大豆蛋白磷酸酶基因GmPP2C72的耐盐功能分析
Analysis of Salt Tolerance Function of Soybean Protein Phosphatase Gene GmPP2C72
摘要
Abstract
In order to clarify the function of the protein phosphatase 2C gene GmPP2C72 in salt stress response and its salt-tolerance molecular mechanism,bioinformatics analysis of GmPP2C72 protein was conducted,GmPP2C72 gene was cloned to construct the overexpression vector,and then was transferred into Lotus japonicus using the cotyle-don node genetic transformation method mediated by Agrobacterium tumefaciens.Finally,the phenotype,physiological indicators and the expression levels of salt tolerance related genes of transgenic plants were analyzed under salt stress condition.The results showed that GmPP2C72 gene contained an open reading frame of 1 206 bp,encoding 401 amino acids,and the encoded protein belonged to the member of the class A subfamily of PP2C family.GmPP2C72 gene did not exhibit tissue expression specificity,it was expressed in various tissues of soybean,and the expression of this gene was induced by salt stress.The overexpression vector of GmPP2C72 gene was successfully constructed.GmPP2C72 gene was successfully transferred into Lotus japonicus,and three transgenic lines with GmPP2C72 gene were success-fully obtained.Compared with wild type,the three transgenic lines maintained better growth state,with significantly higher contents of chlorophyll and proline and lower relative plasma membrane permeability and malondialdehyde content in leaves of Lotus japonicus under salt stress.In addition,the expression levels of four key salt tolerance re-lated genes,LjLEA,LjCOR,LjRD29B,and LjP5CS were significantly higher compared with wild type.Overall,the overexpression of GmPP2C72 gene enhances the salt tolerance of Lotus japonicus by reducing the degree of mem-brane lipid peroxidation and the permeability of the plasma membrane,increasing the contents of chlorophyll and proline in the leaves,and enhancing the expression levels of salt tolerance related genes.关键词
大豆/2C类蛋白磷酸酶(PP2C)/百脉根/耐盐性Key words
Soybean/Protein phosphatase 2C(PP2C)/Lotus japonicus/Salt tolerance分类
农业科技引用本文复制引用
柯丹霞,周兆源,侯仕博,张可馨,宋晓莉,林佳诺..大豆蛋白磷酸酶基因GmPP2C72的耐盐功能分析[J].华北农学报,2026,41(2):12-20,9.基金项目
国家自然科学基金项目(U1904102) （U1904102）
信阳师范大学"南湖学者奖励计划"青年项目(2021) （2021）
