华北农学报2026，Vol.41Issue(2)：28-35,8.DOI:10.7668/hbnxb.20195818
甜樱桃PavJAZ1基因鉴定、克隆及在果实中的表达分析
Identification,Cloning and Expression Analysis of PavJAZ1 in the Fruit of Prunus avium L.
摘要
Abstract
Jasmonates play a crucial role in regulating fruit ripening and quality formation.The JAZ proteins are key repressors of the jasmonic acid signaling pathway.To investigate the function of PavJAZl in sweet cherry fruit ripening and quality regulation,the PavJAZ1 gene was identified and cloned from the sweet cherry genome.Bioinfor-matics analysis was performed to explore its physicochemical properties,protein structure,conserved domains,and cis-acting elements in the promoter region.The gene expression pattern of PavJAZ1 in fruits was analyzed by Quanti-tative Real-time PCR.The subcellular localization of the PavJAZ1 protein was observed,and the interaction between PavJAZ1 and PavMYC2 was verified using the bimolecular fluorescence complementation(BiFC).The results of gene identification and cloning showed that the open reading frame of the PavJAZ1 gene was 837 bp in length,enco-ding 278 amino acids.The molecular weight of the PavJAZ1 was 30.09 ku,the theoretical isoelectric point was 9.21,the instability index was 54.14,and the grand average of hydropathicity was-0.564,indicating that it was an alkaline hydrophilic and unstable properties.Protein structure analysis revealed that PavJAZ1 was mainly composed of random coils and α-helices,containing two conserved domains,the Tify domain and the Jas domain.It also exhibi-ted high homology with proteins from other species,among which it showed the highest homology with that of peach,a plant that also belonged to the genus Prunus of the Rosaceae family.Subcellular localization analysis showed that the PavJAZ 1 protein was localized in the nucleus.The expression level of PavJAZ1 increased significantly in the early sta-ges of fruit development and then gradually decreased.This result suggested that PavJAZ1 may play different roles at different developmental stages.In addition,the BiFC results showed that PavJAZ1 interacted with PavMYC2,confir-ming that PavJAZ1 participates in the jasmonic acid signaling pathway.关键词
甜樱桃/JAZ/果实成熟/表达模式/果实品质Key words
Sweet cherry/JAZ/Fruit ripening/Expression pattern/Fruit quality分类
农业科技引用本文复制引用
王未,段续伟,冉江林,张晓明,闫国华,王晶,周宇,吴传宝,冯琛,张开春..甜樱桃PavJAZ1基因鉴定、克隆及在果实中的表达分析[J].华北农学报,2026,41(2):28-35,8.基金项目
国家自然科学基金项目(32402484,32372664) （32402484,32372664）
北京市农林科学院协同创新专项(KJCX20240408) （KJCX20240408）
