华北农学报2026，Vol.41Issue(2)：36-46,11.DOI:10.7668/hbnxb.20196258
杜梨PbKRP2序列特征与表达特性分析
Analysis of Sequence Characteristics and Expression Patterns of PbKRP2 in Pyrus betulaefolia
摘要
Abstract
The Kip-related protein(KRP)gene family acts as a key regulator of the cell cycle,modulating cell division and endoreduplication by inhibiting cyclin-dependent kinase(CDK)activity,and participates in plant abiot-ic stress responses.To investigate the structural characteristics and environmental stress response profile of PbKRP2 in Pyrus betulifolia,the gene was cloned using reverse transcription PCR(RT-PCR).Bioinformatic analysis was con-ducted to elucidate its sequence features,predicted protein interactions,and phylogenetic relationships.Quantitative Real-time PCR(qRT-PCR)was employed to assess the tissue-specific expression characteristics of PbKRP2 and its expression patterns under various stress conditions.Results showed that PbKRP2 was 585 bp in length,contained 4 exons and 3 introns,and encoded a 194-amino acid protein.The predicted protein had 25 potential phosphorylation sites,a O-glycosylation site,and a conserved C-terminal cyclin-dependent kinase inhibitor(CDI)domain.Phyloge-netic analysis indicated that PbKRP2 was closely related to PyKRP2 in Prunus yedoensis var.nudiflora,PpKRP2 in Prunus persica,and MdKRP2 in Malus domestica.Protein interaction network screening identified potential interac-tions between PbKRP2 and 10 proteins.Analysis of the static structure of potential interacting proteins suggested that PbKRP2 formed spatial interaction conformations with its target proteins PbCDKB1;1 and PbCYCD7;1,respec-tively.The promoter region of PbKRP2 contained multiple cis-acting regulatory elements responsive to environmental factors and hormonal signals.qRT-PCR analysis demonstrated that PbKRP2 transcript levels were significantly high-er in stems and leaves compared to roots,petals,and fruits.Furthermore,PbKRP2 transcription levels were consist-ently upregulated under low-temperature stress and abscisic acid(ABA)treatment,but significantly downregulated under dehydration and salt stress.In summary,these findings provide a candidate gene for stress-resistant breeding of pear and genetic improvement of fruit trees by elucidating the sequence characteristics of PbKRP2 and its differ-ential response patterns to abiotic stresses.关键词
杜梨/PbKRP2基因/基因克隆/非生物胁迫/表达分析Key words
Pyrus betulaefolia/PbKRP2 gene/Gene cloning/Abiotic stress/Expression analysis分类
农业科技引用本文复制引用
靳丛,徐连理,刘琦,张晓小,张宇双,徐源,陈钰祺,任雅楠,王纪忠..杜梨PbKRP2序列特征与表达特性分析[J].华北农学报,2026,41(2):36-46,11.基金项目
国家自然科学基金青年基金项目(31801829) （31801829）
江苏省自然科学基金青年基金项目(BK20170463) （BK20170463）
江苏省大学生创新训练项目(S202511049090) （S202511049090）
淮阴工学院博士科研启动基金项目(Z301B16532) （Z301B16532）
