华北农学报2026，Vol.41Issue(2)：56-62,7.DOI:10.7668/hbnxb.20196002
FaesANT基因参与同型长花柱甜荞花发育的功能分析
Functional Analysis of FaesANT Gene Involved in Floral Development in Long-homostyle Common Buckwheat
摘要
Abstract
Research on the expression pattern and function of FaesANT gene in long-homostyle(LH)common buck-wheat and uncovering its roles involved in floral organ development will help to provide gene resources for the molecular breeding and germplasm innovation of buckwheat.The full-length FaesANT gene was isolated from long-homostyle com-mon buckwheat by combining PacBio SMRT(Single Molecule Real-Time)sequencing and RACE technique.FaesANT ex-pression levels in different tissues and floral buds at sequential developmental stages of long-homostyle common buck-wheat were separately detected by using paraffin sections and qRT-PCR.Moreover,the FaesANT gene function involved in floral development was investigated by using virus-induced gene silencing(VIGS)in long-homostyle common buckwheat.The results were as follows,FaesANT cDNA(1 982 bp)comprised a 1 632 bp ORF encoding a 543 amino acid transcrip-tion factor.Phylogenetic tree analysis grouped the FaesANT into the eudicots ANT lineage.FaesANT expression levels were mainly detected in stamen,pistil and young fruit of long-homostyle common buckwheat and showed no significant differences among them.However,only weak transcripts were detected in the stems,leaves and tepals.During floral bud differentiation,obvious expression of FaesANT gene was detected when pistil and stamen primordia formation,and Fae-sANT expression level reached peak during filament elongation stage of stamen.Then,FaesANT expression decreased gradually with the floral bud development and dropped to the lowest level after flowering.TRV2-FaesA NT-treated long-ho-mostyle plants with strong phenotypic changes produced flowers with style-and filament-length decreased,and stamen number reduced.In addition,some TRV2-FaesANT-treated long-homostyle plants displayed part stamens with anther ho-meotically converted into tepaloid structure.Moreover,FaesANT expression decreased significantly in the flowers of FaesANT-silenced long-homostyle plants with strong phenotypic changes.It suggested that FaesANT was mainly involved in regulating filament and style length,and determining stamen number of common buckwheat based on the gene expres-sion pattern and phenotypes of VIGS-treated long-homostyle common buckwheat flowers.关键词
甜荞/ANT同源基因/基因表达/基因沉默/同型长花柱Key words
Fagopyrum esculentum/ANT homologous gene/Gene expression/Gene silence/Long homostyle分类
农业科技引用本文复制引用
刘钰珍,杨清玉,刘志雄..FaesANT基因参与同型长花柱甜荞花发育的功能分析[J].华北农学报,2026,41(2):56-62,7.基金项目
国家自然科学基金项目(32570432) （32570432）
