河北中医药学报2026，Vol.41Issue(2)：40-45,54,7.
益智仁调节SYK/CARD9/NF-κB信号通路抑制单侧输尿管梗阻大鼠肾纤维化的机制研究
Mechanism of Alpinia Oxyphyllae Fructus in Modulating SYK/CARD9/NF-κB Signaling Pathway to Inhibit Renal Fibrosis in Rats with Unilateral Ureteral Obstruction
摘要
Abstract
Objective:To investigate the inhibitory effects of Alpinia Oxyphyllae Fructus(AOF)on renal fibrosis in rats with unilateral ureteral obstruction(UUO)via SYK/CARD9/NF-κB signaling pathway,and to elucidate the underlying mechanisms.Methods:Thirty male Wistar rats were randomly divided into the sham surgery group(Sham group),UUO group,low-dose AOF group(AOF-L group),medium-dose AOF group(AOF-M group),and high-dose AOF group(AOF-H group).A UUO model was established by unilateral ureteral ligation.Starting on the day of surgery,rats in each AOF dose group were administered the corresponding dose of AOF concentrated decoction via gavage once daily for 21 consecutive days.Tissue samples were collected on day 21 after the surgery.Biochemical assays were used to measure blood urea nitrogen(BUN)and serum creatinine(SCr)in rats from each group;hematoxylin-eosin(HE)and Masson's staining were used to observe histological changes;immunohistochemistry was used to detect the expression of transforming growth factor-β1(TGF-β1),α-smooth muscle actin(α-SMA),and fibronectin(FN);and Western blot analysis was used to assess the expression levels of proteins related to the SYK/CARD9/NF-κB signaling pathway.Results:Compared with the Sham group,the UUO group exhibited elevated SCr and BUN(P＜0.05).Histopathological examination revealed widespread tubular dilation,irregular tubular lumen morphology,epithelial cell degeneration and necrosis,and significant inflammatory cell infiltration in the interstitium.The relative expression levels of signaling pathway-related proteins p-SYK/SYK,CARD9,p-NF-κB p65/NF-κB p65,and renal fibrosis-associated proteins TGF-β1,α-SMA,and FN were all elevated(P＜0.05).Compared with the UUO group,rats treated with AOF showed reduced SCr and BUN levels(P＜0.05),alleviated renal tissue lesions and collagen deposition,downregulated p-SYK/SYK,CARD9,p-NF-κB p65/NF-κB p65,and reduced TGF-β1,α-SMA,and FN(P＜0.05).Furthermore,changes in these indicators were dose-dependent,with the most significant improvement observed in the AOF-H group(P＜0.05).Conclusion:AOF can improve renal fibrosis in UUO rats,and its mechanism of action may be related to the inhibition of the SYK/CARD9/NF-κB pathway.关键词
益智仁/肾纤维化/单侧输尿管梗阻/脾酪氨酸激酶/半胱氨酸酶募集结构域蛋白9/核因子-κBKey words
AOF/renal fibrosis/unilateral ureteral obstruction/spleen tyrosine kinase/cystatin-recruiting domain protein 9/nuclear factor-κB分类
医药卫生引用本文复制引用
任姝畅,邹艳红..益智仁调节SYK/CARD9/NF-κB信号通路抑制单侧输尿管梗阻大鼠肾纤维化的机制研究[J].河北中医药学报,2026,41(2):40-45,54,7.基金项目
黑龙江省教育厅科研基础研究项目(2020-KYYWF-0286) （2020-KYYWF-0286）
