江苏农业学报2026，Vol.42Issue(4)：659-669,11.DOI:10.3969/j.issn.1000-4440.2026.04.002
OsCERK1DY基因导入籼稻中与丛枝菌根(AM)真菌的共生效应
Symbiotic effect of the OsCERK1DY gene introduced into indica rice with arbuscular mycorrhizal(AM)fungi
摘要
Abstract
In this study,using the indica rice restorer line Chunhui 350 as the control and the single-segment substi-tution line Chunhui 356,which carries the arbuscular mycorrhizal(AM)fungus high-efficiency symbiosis gene Os-CERK1DY,as the experimental material,four phosphorus(P)fertilizer application levels were set:0 kg/hm2(P0),50%of the conventional phosphorus application rate(P50),75%of the conventional phosphorus application rate(P75),and the conventional phosphorus application rate(P100,with P2 O5 application at 75 kg/hm2).The symbiotic effect of the OsCERK1DY gene with arbuscular mycorrhizal(AM)fungi in indica rice was evaluated.The results showed that,compared with Chunhui 350,the AM fungal colonization rate in the roots of Chunhui 356 was signifi-cantly increased.Overall,under different phosphorus fertilizer levels in the field,its chlorophyll content,dry matter weight,phosphorus uptake,and phosphorus fertilizer use efficiency were higher than those of Chunhui 350.Results of the two-year field experiment showed that the effective panicle number of Chunhui 356 was significantly higher than that of Chunhui 350 under all phosphorus fertilizer levels(P<0.05).Under the three treatments P0,P50,and P75,the actual yield of Chunhui 356 was significantly higher than that of Chunhui 350(P<0.05).Analysis of the rice rhizosphere soil mi-crobial diversity showed that,compared with Chunhui 350,the rhizosphere soil of Chunhui 356 exhibited higher species richness and diversity,with enrichment of more bacteria from the genera Chryseobacterium and Bradyrhizobium.The relative abundances of functional genes related to hydrocarbon degradation and nitrogen fixation were significantly(P<0.05)or ex-tremely significantly(P<0.01)increased.In conclusion,introducing the OsCERK1DY gene into indica rice can produce beneficial symbiotic effects and can be used to breed rice cultivars with high nutrient use efficiency.关键词
OsCERK1DY基因/丛枝菌根(AM)真菌/籼稻/共生效应Key words
OsCERK1DY gene/arbuscular mycorrhizal(AM)fungi/indica rice/symbiotic effect分类
农业科技引用本文复制引用
杨宙,邹国兴,韩瑞才,朱珊,汤国平,邓伟,黄仁良..OsCERK1DY基因导入籼稻中与丛枝菌根(AM)真菌的共生效应[J].江苏农业学报,2026,42(4):659-669,11.基金项目
江西省农业科学院基础研究与人才培养专项(JX-SNKYJCRC202316) （JX-SNKYJCRC202316）
国家红壤改良工程技术研究中心开放基金课题(2020NETRCRSI-7) （2020NETRCRSI-7）
江西省主要学科学术和技术带头人培养项目(20232BCJ22014) （20232BCJ22014）
