江苏农业学报2026，Vol.42Issue(4)：682-690,9.DOI:10.3969/j.issn.1000-4440.2026.04.004
超甜玉米籽粒维生素C含量关联位点定位及基因挖掘
Association mapping and gene mining for vitamin C content in super-sweet corn kernels
摘要
Abstract
To uncover the regulatory mechanisms underlying the accumulation of nutrients in the kernels of super-sweet corn,this study employed the BLINK model to identify the major loci regulating vitamin C content in super-sweet corn kernels and predict the candidate genes.The results showed that the vitamin C content in super-sweet corn kernels ranged from 0.156 mg/g to 1.289 mg/g,with an average of 0.749 mg/g,exhibiting a normal distribution.A genome-wide association study(GWAS)identified six single nucleotide polymorphism(SNP)loci significantly associated with kernel vitamin C content,with the phenotypic variation explained(PVE)ranging from 9.53%to 12.83%,among which the Affx-91328273 locus had the highest PVE.A total of 153 candidate genes were identified within the 200 kb confidence interval upstream and downstream of the significant SNP loci,of which 58 were annotated genes,accounting for 37.9%.Six genes including GRMZM2G093098,GRMZM2G106061,GRMZM2G179308,GRMZM2G101274,GRMZM2G004222 and GRMZM2G133631 were identifed as key genes regulating kernel vitamin C content in super-sweet corn.These genes were mainly involved in cell division,signal trans-duction,energy metabolism and other pathways.These findings provide important support for an in-depth understanding of the genetic mechanisms underlying kernel vitamin C content in super-sweet corn.关键词
超甜玉米/维生素C/全基因组关联分析/候选基因Key words
super-sweet corn/vitamin C/genome-wide association study/candidate genes分类
农业科技引用本文复制引用
邓召,崔妮妮,余育林,文伟祺,程昕昕,余海兵..超甜玉米籽粒维生素C含量关联位点定位及基因挖掘[J].江苏农业学报,2026,42(4):682-690,9.基金项目
安徽省高校自然科学研究重大项目(KJ2019ZD57) （KJ2019ZD57）
安徽科技学院科研发展基金项目(FZ230126) （FZ230126）
安徽省功能农业与功能食品重点实验室(安徽科技学院)项目(iFAST-2024-3) （安徽科技学院）
安徽科技学院作物学高峰学科基金项目(XK-XJGF001) （XK-XJGF001）
