扬州大学学报（农业与生命科学版）2026，Vol.47Issue(2)：23-32,10.DOI:10.16872/j.cnki.1671-4652.2026.02.003
副溶血弧菌噬菌体VpP2326的分离鉴定及表征
Isolation,identification and characterization of a Vibrio parahaemolyticus phage VpP2326
摘要
Abstract
To promote the development of phage-based technologies for the prevention and control of Vibrio parahae-molyticus,a phage was isolated and purified by the double-layer agar plate method with V.parahaemolyticus RIMD2210633 strain as the host bacterium.Thereafter,its biological characteristics were explored.The results showed that the isolated phage VpP2326 had an icosahedral head and a short tail.It exhibited a significant antibacterial effect on the host bacterium and a strong host specificity.The optimal multiplicity of infection(MOI)of this phage was 0.1,the incubation period was less than 5 min,and the burst size was approximately 147 PFU·cell-1.VpP2326 had good thermal and pH stabili-ty,was sensitive to chloroform and proteases,and was highly sensitive to UV radiation.The nucleic acid type of this phage was double-stranded DNA,the whole genome was approximately 43 kb,with the G+C content of 49.37％,46 open reading frames are predicted in the genome.This study laid the foundation for the development and application of VpP2326.关键词
副溶血弧菌/噬菌体/生物学特性/全基因组测序Key words
Vibrio parahaemolyticus/phage/biological characteristics/whole genome sequencing分类
生物科学引用本文复制引用
张玙,王梦娴,徐艳华,吴双,朱晓雅,徐正中,陈祥,焦新安,郑成坤..副溶血弧菌噬菌体VpP2326的分离鉴定及表征[J].扬州大学学报（农业与生命科学版）,2026,47(2):23-32,10.基金项目
国家自然科学基金资助项目(31802210) （31802210）
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
江苏省大学生创新创业训练计划项目(202411117082Z) （202411117082Z）
