扬州大学学报（农业与生命科学版）2026，Vol.47Issue(2)：41-50,121,11.DOI:10.16872/j.cnki.1671-4652.2026.02.005
基于转录组学探究金鱼早期发育过程中脂代谢相关基因的表达
Exploring lipid metabolism-related gene expression during early goldfish development via transcriptomics
摘要
Abstract
To elucidate the expression dynamics of lipid metabolism-related genes during early development in goldfish(Carassius auratus),we conducted transcriptome sequencing to profile differentially expressed genes(DEGs)at three critical developmental stages(0 h,48 h,and 5 d post-fertilization)in two distinct goldfish varieties(Oranda and Pearl-scale).Subsequent qRT-PCR validation confirmed the transcriptomic findings.Comparative analysis revealed a significant number of DEGs across all developmental transitions in both varieties.Enrichment analysis indicated that downregulated DEGs(48 h vs 0 h,5 d vs 48 h,and 5 d vs 0 h)were predominantly associated with pathways involved in RNA trans-port,pyrimidine metabolism,and oocyte meiosis.In contrast,upregulated DEGs exhibited pathway enrichment exclu-sively in the 5 d vs 48 h comparison,with both varieties showing significant enrichment in the PPAR signaling pathway.Further analysis identified twelve candidate genes including apoba,fads2,pparα,and pparγ as potential key regulators of lipid metabolism during early goldfish development.Collectively,these findings demonstrate pronounced differences in PPAR signaling and lipid metabolism-related gene expression between the 5 d and 48 h stages,supporting the hypothesis that post-hatching goldfish undergo a marked enhancement in lipid metabolic capacity.关键词
金鱼/早期发育/脂代谢/转录组学/差异表达基因Key words
goldfish/early development/lipid metabolism/transcriptomics/differentially expressed gene分类
农业科技引用本文复制引用
李澳,董小敬,秦文皓,杜雪地,王剑桥,赵孟杰,李方希,曹壮,高鹏..基于转录组学探究金鱼早期发育过程中脂代谢相关基因的表达[J].扬州大学学报（农业与生命科学版）,2026,47(2):41-50,121,11.基金项目
国家自然科学基金资助项目(31802310、31702004) （31802310、31702004）
淡水鱼类发育生物学国家重点实验室资助项目(2019KF006) （2019KF006）
