昆虫学报2026，Vol.69Issue(3)：387-398,12.DOI:10.16380/j.kcxb.2026.03.007
蜡蚧轮枝菌JMC-01菌株的内生定殖对甘蓝的促生作用及诱导的甘蓝挥发物对桃蚜的驱避效果
Growth-promoting effect of endophytic colonization of Lecanicillium lecanii strain JMC-01 in cabbage(Brassica oleracea var.capitata)and the repellent effects of induced cabbage volatiles against Myzus persicae(Hemiptera:Aphididae)
摘要
Abstract
[Aim]This study aims to explore the endophytic colonization potential of Lecanicillium lecanii strain JMC-01 in cabbage(Brassica oleracea var.capitata),its physiological impacts on the host plant and the repellent effects of induced cabbage volatile compounds against Myzus persicae.[Methods]The L.lecanii strain JMC-01 spore suspensions at the concentrations of 1 × 108,1 × 107 and 1 × 106 spores/mL were inoculated to cabbage plants using the leaf spraying method and root drenching method,respectively.The colonization rates were determined using the PDA plate detection method on the 1 st,3rd,7th,10th,14th and 21st day after inoculation.The growth promoting effect of L.lecanii strain JMC-01 on cabbage was investigated by measuring the plant height,root length,fresh weight,dry weight,stem diameter and chlorophyll content of the cabbage on the 21st day after inoculation.The insect Y-tube olfactometer was used to detect the behavioral selection of the wingless adults and day-1 1st instar nymphs of M.persicae to the cabbage plants at 24 h after inoculation with the spore suspension of L.lecanii strain JMC-01 at the concentration of 1 × 108 spores/mL.Gas chromatography-mass spectrometry(GC-MS)was used to identify differential volatile compounds from the cabbage inoculated and uninoculated with the spore suspension of L.lecanii strain JMC-01 at the concentration of 1 × 108 spores/mL.The insect Y-tube olfactometer was used to detect the repellent effects of these differential volatile compounds induced by L.lecanii strain JMC-01 against the wingless adults and day-1 1st instar nymphs of M.persicae.[Results]The L.lecanii strain JMC-01 inoculated with leaf spraying method exhibited superior colonization efficiency in cabbage plants to that with root drenching method,achieving 100％colonization rates in cabbage across all the three concentrations on the 1st day post inoculation with leaf spraying method,with detectable colonization signals persisting on the 21st day after treatment with L.lecanii strain JMC-01 spore suspension at the concentration of 1 × 107 spores/mL.Growth promotion experiments demonstrated that L.lecanii strain JMC-01 spore suspensions at the concentrations of 1 × 108 and 1 × 107 spores/mL significantly enhanced the plant height,root length,stem diameter and biomass accumulation,with the L.lecanii strain JMC-01 spore suspension at the concentration of 1 × 107 spores/mL showing the most pronounced promoting effect on chlorophyll synthesis.The total chlorophyll content in the cabbage treated with L.lecanii strain JMC-01 spore suspension at the concentration of 1 × 107 spores/mL reached 37 mg/g.The L.lecanii strain JMC-01 spore suspension treatment on the cabbage plants exhibited repellent effects on the wingless adults and day-1 1st instar nymphs of M.persicae.GC-MS analysis identified 12 differential volatile organic compounds induced by L.lecanii strain JMC-01 colonization in cabbage,among which n-hexadecane and 3-methyl-2-oxyindole exhibited repellent effects on the wingless adults and day-1 1st instar nymphs of M.persicae.The wingless adult M.persicae showed stronger olfactory response to the standard substances n-hexadecane and 3-methyl-2-oxyindole than the day-1 1st instar nymphs.The repellent effect of n-hexadecane on the day-1 1st instar nymphs increased with the spore suspension concentration increasing.[Conclusion]This study reveals that the L.lecanii strain JMC-01 can endophytically colonize in the cabbage plants,and can achieve a growth-promoting effect on the cabbage through endophytic colonization.At the same time,it can also induce the plant to produce volatile compounds to repel M.persicae,providing a theoretical basis for the application of entomopathogenic fungi in biological control strategies.关键词
桃蚜/蜡蚧轮枝菌/甘蓝/内生定殖/促生作用/驱避作用Key words
Myzus persicae/Lecanicillium lecanii/Brassica oleracea var.capitata/endogenous colonization/growth promotion/repellent effect分类
生物科学引用本文复制引用
颜晓曦,田浩然,吕娜玉,李金鹏,洪波,贾彦霞..蜡蚧轮枝菌JMC-01菌株的内生定殖对甘蓝的促生作用及诱导的甘蓝挥发物对桃蚜的驱避效果[J].昆虫学报,2026,69(3):387-398,12.基金项目
宁夏自然科学基金项目(2023AAC03073) （2023AAC03073）
宁夏重点研发计划项目(2024BBF02025) （2024BBF02025）
2025年宁夏大学研究生创新项目(CXXM2025055) （CXXM2025055）
