临床眼科杂志2026，Vol.34Issue(2)：104-109,6.DOI:10.3969/j.issn.1006-8422.2026.02.002
薯蓣皂苷元调节PINK1/Parkin通路对高糖诱导的视网膜血管内皮细胞损伤的影响
Effect of diosgenin on high glucose induced retinal endothelial cell damage by modulating PINK1/Parkin pathway
摘要
Abstract
Objective To investigate the effect of diosgenin(DSG)on high glucose(HG)-induced damage to hu-man retinal capillary endothelial cells(HRCE)by modulating the PTEN-induced kinase 1(PINK1)/Parkin pathway.Methods HRCE cells were assigned to the control group,HG group,HG+DSG(100 μg/mL)group,and HG+DSG+au-tophagy inhibitor(Mdivi-1,25 μm)group.The CCK-8 method was applied to test the proliferation ability of HRCE cells.Flow cytometry was performed to test cell apoptosis.Transmission electron microscopy was applied to observe cellular au-tophagy.Fluorescent probe was performed to test reactive oxygen species(ROS)activity.Enzyme-linked immunosorbent as-say(ELISA)experiment was used to measure tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in the cell super-natant,and the activity of superoxide dismutase(SOD).Western blot experiments were used to detect PINK1/Parkin path-way related proteins in HRCE cells.Results Compared to the control group,the HG group showed an increase in the num-ber of mitochondrial fragments and a decrease in the number of autophagosomes in HRCE cells,and the cell survival rate,SOD activity,PINK1,Parkin,and Beclin1 proteins reduced manifestly(all P<0.05),while cell apoptosis rate,ROS ac-tivity,TNF-α and IL-6 levels increased clearly(all P<0.05).Compared to the HG group,the HG+DSG group showed a decrease in the number of mitochondrial fragments and an increase in the number of autophagosomes and autolysosomes,and the cell survival rate,SOD activity,PINK1,Parkin,and Beclin1 proteins increased clearly(all P<0.05),while cell apoptosis rate,ROS activity,TNF-α,and IL-6 levels reduced manifestly(all P<0.05).Compared to the HG+DSG group,the HG+DSG+Mdivi-1 group showed an increase in the number of mitochondrial fragments in cells and a decrease in the number of autophagosomes,the cell survival rate,SOD activity,PINK1,Parkin,and Beclin1 proteins reduced manifestly(all P<0.05),while cell apoptosis rate,ROS activity,TNF-α,and IL-6 increased clearly(all P<0.05).Conclusions DSG may promote proliferation and autophagy of HRCE cells,inhibit apoptosis,reduce inflammatory cytokine secretion,and alleviate oxidative stress damage by modulating PINK1/Parkin pathway proteins induced by HG.关键词
薯蓣皂苷元/PTEN诱导激酶1/Parkin通路/视网膜血管内皮细胞Key words
Diosgenin/PTEN-induced kinase 1/Parkin pathway/Human retinal endothelial cells引用本文复制引用
都艳红,张士宏,夏春晓,王艳新..薯蓣皂苷元调节PINK1/Parkin通路对高糖诱导的视网膜血管内皮细胞损伤的影响[J].临床眼科杂志,2026,34(2):104-109,6.基金项目
河北省保定市科技计划项目(2141ZF218) （2141ZF218）
