临床眼科杂志2026，Vol.34Issue(2)：110-115,6.DOI:10.3969/j.issn.1006-8422.2026.02.003
小檗碱调节ROS/TXNIP/NLRP3通路对高糖诱导的视网膜神经节细胞凋亡的影响
Impact of berberine on high glucose induced apoptosis of retinal ganglion cells by regulating the ROS/TXNIP/NLRP3 pathway
摘要
Abstract
Objective To investigate the impact of berberine on high glucose induced apoptosis of retinal gan-glion cells(RGCs)by regulating reactive oxygen species(ROS)/thioredoxin-binding protein(TXNIP)/NOD-like receptor pyrin domain containing 3(NLRP3)pathway.Methods RGC-5 cells were assigned into the control group,high glucose group,berberine group,negative control plasmid(pcDNA-NC)group,TXNIP overexpression plasmid(pcDNA-TXNIP)group,and berberine+pcDNA-TXNIP group.Cell viability was detected using the Cell Counting Kit(CCK-8)method.Cell apoptosis rate was detected using flow cytometry.The levels of malondialdehyde(MDA),glutathione peroxidase(GSH-Px),superoxide dismutase(SOD),interleukin-1β(IL-1β),and interleukin-18(IL-18)in cell lysate were detected us-ing enzyme-linked immunosorbent assay(ELISA).The detection of ROS was performed using the 2',7'-Dichlorodihydro-fluorescein diacetate(DCFH-DA)method.Western blot was carried out to measure the expression of Bcl-2 associated X protein(Bax),Caspase-3,Caspase-9,TXNIP,and NLRP3 proteins.Results The high glucose group showed a decrease in optical density at 450 nm(OD450)value,GSH-Px,and SOD levels in RGC-5 cells,and an increase in apoptosis rate,MDA,IL-1β,IL-18,ROS levels,and Bax,Caspase-3,Caspase-9,TXNIP,and NLRP3 protein expression levels than the control group,and all differences were statistically significant(all P<0.05).The berberine group showed an increase in OD450 value,GSH-Px,and SOD levels in RGC-5 cells,and a decrease in apoptosis rate,MDA,IL-1β,IL-18,ROS levels and the apoptosis and TXNIP/NLRP3 pathway protein expression compared with the high glucose group,and all differences were statisticallg significant(all P<0.05);while the pcDNA-TXNIP group showed a decrease in OD450 value,GSH-Px,and SOD levels in RGC-5 cells,and an increase in apoptosis rate,MDA,IL-1β,IL-18,ROS levels,and the apoptosis protein and TXNIP/NLRP3 pathway protein expression(all P<0.05).The berberine+pcDNA-TXNIP group had a decrease in OD450 value,GSH-Px,and SOD levels in RGC-5 cells,and an increase in apoptosis rate,MDA,IL-1β,IL-18,ROS levels,and the apoptosis protein and TXNIP/NLRP3 pathway protein expression compared with the berberine group(all P<0.05).Conclusions Berberine can inhibit high glucose induced apoptosis of RGCs by suppressing the ROS/TXNIP/NLRP3 pathway.关键词
活性氧/硫氧还蛋白结合蛋白/NOD样受体相关蛋白3/小檗碱/高糖/视网膜神经节细胞Key words
Reactive oxygen species/Thioredoxin-binding protein/NOD-like receptor pyrin domain containing 3/Berberine/High glucose/Retinal ganglion cells引用本文复制引用
高毓妍,高惠英..小檗碱调节ROS/TXNIP/NLRP3通路对高糖诱导的视网膜神经节细胞凋亡的影响[J].临床眼科杂志,2026,34(2):110-115,6.基金项目
河北省医学科学研究重点课题计划项目(20230227) （20230227）
