南京理工大学学报（自然科学版）2026，Vol.50Issue(2)：143-151,9.DOI:10.14177/j.cnki.32-1397n.2026.50.02.004
基于微流控技术的ELISA检测器件常见衬底蛋白孵育方法的探究及应用
Exploration and application of common substrate protein incubation methods for ELISA detection device based on microfluidic technology
摘要
Abstract
In order to study the protein binding ability of substrates commonly used in microfluidic devices,to optimize enzyme-linked immunosorbent assay(ELISA)with microfluidic technology and further optimize assay time,cost and sample size required,this paper explores and summarizes the methods to enhance the protein binding ability of glass substrates and lithium niobate wafers,and the results show that 3-aminopropyl triethoxysilane(3-APTES)can increase the number of amino acids on the surface of substrates and promote the binding of substrates to proteins.On this basis,this paper proposes a droplet-based ELISA detection method that combines the 3-APTES-treated glass substrate with microdroplets to accomplish SARS-CoV-2(2019-nCoV)antibody detection with the assistance of acoustic streaming,which reduces the amount of required samples by 95%,reducing the antibody incubation time to 40 s,revealing the potential of microfluidic devices for immunoassay applications.关键词
酶联免疫吸附测定/微流控/衬底处理/蛋白吸附/免疫检测Key words
enzyme-linked immunosorbent assay/microfluidics/substrate treatment/protein binding/immunoassay分类
信息技术与安全科学引用本文复制引用
王琪,宋建超..基于微流控技术的ELISA检测器件常见衬底蛋白孵育方法的探究及应用[J].南京理工大学学报（自然科学版）,2026,50(2):143-151,9.基金项目
国家自然科学基金(62531002) （62531002）
中央高校基本科研业务费专项资金(30925010605 （）
30925010540 （）
30924012001) （）
