上海海洋大学学报2026，Vol.35Issue(3)：731-739,9.DOI:10.12024/jsou.20240804610
鳗弧菌减毒菌灭活疫苗对花鲈免疫效果的研究
Study on the immune effect of inactivated vaccine of Vibrio anguillarum attenuated to Lateolabrax maculate
摘要
Abstract
In order to study the vibrio anguillarum minus toadstool inactivated vaccine immune effect,this study attenuated strains of V.anguillarum Δvah1-vah4-rtxA for high temperature inactivated bacteria fermentation preparation into inactivated vaccines,2％according to the proportion of added to the feed,oral immune Lateolabrax maculatus 21 d.Blood was collected from the caudal vertebral vein at 0 d,7 d,14 d and 21 d after immunization,and the serum levels of immunoglobulin M(IgM)and lysozyme(LZM)were measured.After 21 days of immunization,the fish were challenged with wild type V.anguillarum via intraperitoneal injection.The results showed that compared with the control group,IgM content in the serum of the immunized group of L.maculatus increased significantly and reached the highest value by the 21st day.Serum LZM content increased to reach the highest value by the 14th d,and thereafter,decreased slightly.Transcriptome analysis of the head kidney of L.maculatus for 21 d of immunization showed 252 significantly up-regulated genes and 200 significantly down-regulated genes compared to the control group,and KEGG analysis showed that these differential genes were significantly enriched for hematopoietic cell spectrum,natural killer cell-mediated cytotoxicity,phagolysosomes,the NF-κB signaling pathway,the production of IgA by intestinal immune network,and the Fc epsilon RI and other pathways.The relative immunoprotection rates at 7 d and 14 d were 62.5％and 25.0％,respectively.Pathological observations of the immunized group showed that the extent of vacuolization of the intestinal mucosa and structural damage to the gill tissues were relatively mild.The above results indicated that the attenuated strain of V.anguillarum Δvah1-vah4-rtxA preparation into inactivated vaccine,to improve the flower L.maculatus against V.anguillarum infection have better effect.关键词
鳗弧菌/花鲈/减毒菌株/灭活疫苗/免疫保护率Key words
Vibrio anguillarum/Lateolabrax maculatus/attenuated strain/inactivated vaccine/immunoprotection rate分类
农业科技引用本文复制引用
王生明,谢海生,丁长晨,陈小辉,罗土炎,鲍宝龙..鳗弧菌减毒菌灭活疫苗对花鲈免疫效果的研究[J].上海海洋大学学报,2026,35(3):731-739,9.基金项目
国家重点研发计划(2022YFD2400100) （2022YFD2400100）
福建省促进海洋与渔业产业高质量发展专项(FJHYF-L-2023-16) （FJHYF-L-2023-16）
福建省区域发展项目(2021N3016) （2021N3016）
