药学研究2026，Vol.45Issue(4)：381-387,415,8.DOI:10.13506/j.cnki.jpr.2026.04.004
基于 UPLC-Q-TOF-MS 的泽兰体内外化学成分解析及其抗卵巢癌的网络药理学机制研究
Investigation of the chemical components of Lycopus lucidus in vitro and in vivo based on UPLC-Q-TOF-MS and the network pharmacology mechanism against ovarian cancer
焦柱铖 1包永睿 2王帅 2李天娇 2孟宪生2
作者信息
- 1. 辽宁中医药大学药学院,辽宁 大连 116600
- 2. 辽宁中医药大学药学院,辽宁 大连 116600||辽宁省中药多维分析专业技术创新中心,辽宁 大连 116600||辽宁省现代中药研究工程实验室,辽宁 大连 116600
- 折叠
摘要
Abstract
Objective To systematically analyze the chemical components of Lycopus lucidus and its blood-absorbed components,thereby providing a theoretical basis for elucidating the pharmacodynamic material basis of its anti-ovarian cancer effects.Methods UPLC-Q-TOF-MS was employed to characterize and identify components in the Lycopus lucidus extract,blank plasma,and drug-containing plasma,by consulting literature and databases and analyzing secondary fragment ions.The SwissTargetPrediction and GeneCards databases were used to retrieve and screen the targets of the blood-absorbed components against ovarian cancer,aiming to clarify the pharmacodynamic material basis.Finally,SKOV-3 ovarian cancer cells were treated with different concentrations of the extract to evaluate its impact on cell viability.Results By comparing the extract,blank plasma,and drug-containing plasma under identical analytical conditions,49 compounds were preliminarily identified from the Lycopus lucidus extract,including flavonoids,terpenoids,and phenolics.Among these,12 components were characterized as blood-absorbed constituents.Based on this,network pharmacology identified 10 key targets related to the anti-ovarian cancer activity of Lycopus lucidus.Furthermore,the Lycopus lucidus extract promoted SKOV-3 cell apoptosis in a dose-dependent manner.Conclusion This study systematically identified the chemical components of the Lycopus lucidus extract and its blood-absorbed constituents.The results indicate that the blood-absorbed components are likely the effective substances responsible for its anti-ovarian cancer effects,further clarifying the pharmacodynamic material basis of Lycopus lucidus against ovarian cancer.关键词
泽兰/入血成分/卵巢癌/SKOV-3 细胞/超高效液相色谱-四极杆飞行时间串联质谱/网络药理学Key words
Lycopus lucidus Turcz.var.hirtus Regel/Blood-absorbed components/Ovarian cancer/SKOV-3 cell/UPLC-Q-TOF-MS/Network pharmacology分类
医药卫生引用本文复制引用
焦柱铖,包永睿,王帅,李天娇,孟宪生..基于 UPLC-Q-TOF-MS 的泽兰体内外化学成分解析及其抗卵巢癌的网络药理学机制研究[J].药学研究,2026,45(4):381-387,415,8.
